Uv Killed Bacteria

UV-killed bacteria are bacterial cells rendered unable to reproduce by exposure to ultraviolet (UV) radiation, providing biologically inactive material for laboratory study. UV photons damage bacterial DNA, commonly creating pyrimidine dimers and other lesions that block DNA replication and transcription, while much of the cell’s physical structure can remain intact. In biology, these cells support investigations of bacterial surface components, immune recognition, and host–microbe interactions without introducing actively growing cultures. They also serve as models for evaluating UV disinfection, although effectiveness depends on radiation dose, exposure time, bacterial species, and access of UV light to the cells.

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JoVE EoE - Immunodiagnostics

A Riboflavin and UV Light Treatment of Platelet Products to Eliminate Bacteria

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2025

This video demonstrates the riboflavin and UV light-based pathogen reduction process against high-titer bacterial contamination in human platelet products. This process could help to lower the risk of severe adverse transfusion events associated with bacterial contamination.

Lysostaphin-Based Enzyme Protection Assay: An In Vitro Method to Quantify Intracellular Staphylococcus aureus Load by Enzyme-Mediated Selective Killing of Extracellular Bacteria

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2025

In this video, we demonstrate a lysostaphin-based in vitro enzyme protection assay to quantify the Staphylococcus aureus internalization in A549 cells. The assay helps in qualitative, quantitative, and characterization studies of the intracellular bacterial load in the host cells.

Assessment of Opsonophagocytic Killing Activity against Bacterial Pathogens

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2025

The video shows an in vitro opsonophagocytic killing assay using a co-culture of human neutrophils with Streptococcus pneumoniae. Antibodies and complement proteins facilitate opsonization of the bacteria, enabling neutrophils to recognize and kill bacteria, showing successful neutrophil opsonophagocytic activity.

In Vitro Evaluation of Bacterial Killing by Neonatal Mouse Splenic Phagocytes

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2026

Source: Seman, B. G., et.al. Neonatal Imaging Model of Gram-Negative Bacterial Sepsis. J. Vis. Exp. (2020)This video demonstrates the isolation of neutrophils and inflammatory monocytes from neonatal mouse spleens and their application in a luminescent bacterial killing assay. It covers mechanical dissociation, red blood cell lysis, magnetic bead–based cell enrichment, bacterial incubation, and antibiotic protection. Timed luminescence readings indicate initial bacterial internalization...

In Vitro Stimulation of Mouse Splenocytes with Heat-Killed Listeria monocytogenes

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2026

Source: Ahn, J. J., et al., Experimental Infection with Listeria monocytogenes as a Model for Studying Host Interferon-γ Responses. J. Vis. Exp. (2016)This video demonstrates the preparation of mouse splenocyte cultures from Listeria monocytogenes-infected mice for downstream cytokine analysis. It includes ex vivo bacteria-derived antigen stimulation and sample processing to study T cell responses.

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