Body Wall Muscle Patch
Body Wall Muscle Patch - Related Videos
08:03
In Vivo Calcium Imaging in C. elegans Body Wall Muscles
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Cited by 3 •
2019
This method provides a way to couple optogenetics and genetically encoded calcium sensors to image baseline cytosolic calcium levels and changes in evoked calcium transients in the body wall muscles of the model organism C. elegans.
07:49
Immunohistological Labeling of Microtubules in Sensory Neuron Dendrites, Tracheae, and Muscles in the Drosophila Larva Body Wall
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Cited by 11 •
2011
To understand how complex cell shapes, such as neuronal dendrites, are achieved during development, it is important to be able to accurately assay microtubule organization. Here we describe a robust immunohistological labeling method to examine microtubule organization of dendritic arborization neuron sensory dendrites, trachea, muscle, and other Drosophila larva body wall tissues.
02:50
Perforated Patch-Clamp Recordings of Inner Ear Sensory Neuron Cell Bodies
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2025
This video demonstrates the patch-clamp technique for measuring ionic currents in inner ear sensory neuron cell bodies. It details the preparation and use of a polished patch-clamp pipette filled with an internal solution containing amphotericin B, which perforates the membrane to allow ion movement while preserving intracellular conditions. Voltage pulses are applied to trigger and record ion channel activity, providing insights into their properties and behavior.
07:29
Paired Patch Clamp Recordings from Motor-neuron and Target Skeletal Muscle in Zebrafish
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Cited by 17 •
2010
Larval zebrafish represent the first vertebrate model system to allow simultaneous patch clamp recording from a spinal motor-neuron and target skeletal muscle. This video demonstrates the microscopic methods used to identify a segmental CaP motor-neuron and target muscle cells as well as the methodologies for recording from each cell type.
09:43
Patch Clamp Electrophysiology
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2023
Neuron cell membranes are populated with ion channels that control the movement of charge into and out of the cell, thereby regulating neuron firing. One extremely useful technique for investigating the biophysical properties of these channels is called patch clamp recording. In this method, neuroscientists place a polished glass micropipette against a cell and apply suction to form a high resistance seal. This process isolates a small “patch” of membrane that contains one or more ion channels.
FAQs
Ion channels regulate the movement of ions across muscle-cell membranes, producing membrane currents that alter electrical excitability. Changes in excitability determine how readily the cells respond to signals associated with movement and contraction. Recording these currents helps investigators connect channel behavior with the muscle’s functional output rather than examining contraction as an isolated event.
Membrane excitability describes how strongly a muscle cell responds to electrical or physiological stimulation. Patch-clamp recordings reveal changes in this property by measuring membrane currents and ion-channel activity. Comparing excitability under different genetic or pharmacological conditions can show how cellular electrical behavior contributes to altered neuromuscular function and movement.
Visible movement shows the final mechanical outcome, whereas patch-clamp analysis examines electrical events inside the muscle cells that precede or support it. By recording ion-channel activity and membrane currents, investigators can identify cellular changes that may explain differences in responsiveness, coordination, or contraction. This provides a mechanistic link between membrane physiology and motor behavior.
The preparation is positioned so investigators can access the relevant muscle cells with a patch-clamp electrode. A seal is formed with the cell membrane, creating a controlled electrical connection for recording ion-channel activity and membrane currents. Maintaining this seal is central to obtaining interpretable measurements of excitability and cellular responses.
Researchers can measure ion-channel activity, membrane currents, and changes in cellular excitability from the muscle preparation. These measurements describe how the cells respond electrically and help relate that response to contraction and movement. Because the system is controlled, investigators can compare conditions after genetic or pharmacological changes and examine their effects on neuromuscular function.
This preparation is useful when a study needs to connect muscle-cell physiology with movement. It supports investigations of cellular physiology, motor control, muscle disorders, and the consequences of genetic or pharmacological changes. Its value comes from combining a localized muscle system with electrophysiological recordings, allowing researchers to analyze mechanisms that influence neuromuscular performance.