Ve Cadherin Junction Proteins
Ve Cadherin Junction Proteins - Related Videos
11:31
Analysis of Protein-protein Interactions and Co-localization Between Components of Gap, Tight, and Adherens Junctions in Murine Mammary Glands
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Cited by 5 •
2017
Intercellular junctions are requisites for mammary gland stage-specific functions and development. This manuscript provides a detailed protocol for the study of protein-protein interactions (PPIs) and co-localization using murine mammary glands. These techniques allow for the investigation of the dynamics of the physical association between intercellular junctions at different developmental stages.
01:25
Structure of Cadherins
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2023
The cadherins were one of the first cell adhesion molecules discovered; the term “cadherins” is based on their calcium-dependent adhering properties. The first cadherins discovered on the epithelial, neuronal, and placental cells were named E-cadherin, P-cadherin, and N-cadherin, respectively. These classical cadherins share sequence and structural similarities. Other cadherins, including those involved in cell signaling, are grouped into non-classical cadherins. This diversity of cadherins...
01:19
Cadherins in Tissue Organization
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2023
The cadherins are a superfamily of cell adhesion molecules comprising over 180 variants, with specific tissues expressing a particular combination of cadherin types. Cadherins generally exhibit homophilic binding; i.e., cadherins on one cell bind to cadherins of the same or closely related type on another cell. Thus, cells of the same type have a specific affinity to bind to each other and sort themselves into clusters to form tissues. Cell Sorting During Development Cell sorting plays an...
04:15
Fluorescence Localization of Tight Junction Proteins and Stress Fibers in Endothelial Cells
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2025
Source: Alluri, H., et al. Oxygen-Glucose Deprivation and Reoxygenation as an In Vitro Ischemia-Reperfusion Injury Model for Studying Blood-Brain Barrier Dysfunction. J. Vis. Exp. (2015).The video demonstrates the fluorescent localization of tight junction proteins and stress fibers in rat brain microvascular endothelial cells (RBMECs). In the stressed monolayer, fluorescently labeled antibodies are used to tag the tight junction proteins, while fluorophore-tagged phalloidin labels the stress...
11:12
Sequential Photo-bleaching to Delineate Single Schwann Cells at the Neuromuscular Junction
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Cited by 10 •
2013
Visualizing individual cells in densely packed tissues, such as terminal Schwann cells (SCs) at neuromuscular junctions (NMJs), is challenging. "Sequential photo-bleaching" allows delineating single terminal SCs, for instance in the triangularis sterni muscle explant, a convenient nerve-muscle preparation, where sequential bleaching can be combined with time-lapse imaging and post-hoc immunostainings.
FAQs
Calcium supports the adhesion function of VE-cadherin at endothelial contacts. Its extracellular domains bind VE-cadherin molecules on neighboring cells, creating a coordinated connection across the junction. This calcium-dependent interaction matters because it helps organize cell-cell contacts rather than treating adhesion as an independent property of individual endothelial cells.
The cytoplasmic regions connect VE-cadherin to catenins and the actin cytoskeleton inside endothelial cells. These associations provide an intracellular structural link that reinforces cell-cell contacts. By coordinating membrane adhesion with the cytoskeletal framework, the junction can remain organized while contributing to the stability of the vascular lining.
Changes in junction organization can alter how readily fluids and molecules move between blood and surrounding tissues. Because VE-cadherin contacts contribute to endothelial barrier control, weakened or disrupted organization is associated with increased vascular leakage and barrier dysfunction. This relationship makes junction integrity important for understanding how vessels regulate exchange with tissues.
VE-cadherin studies provide a way to examine how endothelial cell contacts relate to angiogenesis, the formation of blood vessels. Researchers can connect junction organization and barrier behavior with vascular development processes. This perspective helps place cell-cell adhesion within broader biological questions about how endothelial structures form and function during vessel growth.
Examining VE-cadherin can help relate endothelial junction integrity to the movement of fluids and molecules from blood into tissues. In inflammatory settings, this relationship is relevant because altered barrier control may contribute to vascular leakage. The protein therefore serves as a useful focus for studying how endothelial contacts participate in barrier dysfunction.
VE-cadherin links endothelial cell adhesion with vascular barrier behavior, making it relevant across several biological and medical research areas. Studies can use this connection to investigate vessel integrity in cardiovascular disease, leakage associated with edema, vascular changes in cancer, and endothelial organization during tissue repair. Its significance comes from this shared barrier-related role.