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Neuroscience

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Sequential Photo-bleaching to Delineate Single Schwann Cells at the Neuromuscular Junction
 

Sequential Photo-bleaching to Delineate Single Schwann Cells at the Neuromuscular Junction

Article DOI: 10.3791/4460
January 11th, 2013

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Summary January 11th, 2013

Visualizing individual cells in densely packed tissues, such as terminal Schwann cells (SCs) at neuromuscular junctions (NMJs), is challenging. "Sequential photo-bleaching" allows delineating single terminal SCs, for instance in the triangularis sterni muscle explant, a convenient nerve-muscle preparation, where sequential bleaching can be combined with time-lapse imaging and post-hoc immunostainings.

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