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Immunology and Infection
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Reverse Genetics Mediated Recovery of Infectious Murine Norovirus
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Reverse Genetics Mediated Recovery of Infectious Murine Norovirus

Reverse Genetics Mediated Recovery of Infectious Murine Norovirus

DOI:
10.3791/4145-v

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13:48 min

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June 24, 2012

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, , , ,
1Section of Virology,Imperial College London

Chapters

  • 00:05Title
  • 01:34Procedure Overview
  • 03:16RNA Transcription and Capping for the Recovery of Infectious MNV
  • 06:27MNV Recovery by RNA Electroporation into Raw Cells
  • 08:25Recovery by Lipofection into BSR-T7 Cells
  • 10:07Direct Recovery of Infectious MNV from cDNA in Cells Expressing T7 RNA Polymerase
  • 12:20Representative Results

Summary

Automatic Translation

Automatic Translation

Noroviruses are a major cause of gastroenteritis yet molecular techniques for their characterisation are still relatively new. Here we report two different reverse genetics approaches for the efficient recovery of murine norovirus (MNV), the only member of this genus which can be propagated in cell culture.

Tags

Reverse GeneticsRecoveryInfectiousMurine NorovirusHuman NorovirusesGastroenteritisOutbreaksHospitalsAntiviral ApproachesCell CulturePropagationGenomic RNA MoleculesOpen Reading Frames (ORFs)Non-structural ProteinsCapsid ProteinsVirulence Factor (VF1)

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