Winter Diapause

Winter diapause is a programmed, reversible state of dormancy that allows organisms, especially insects and other arthropods, to survive cold seasons when food and suitable conditions are limited. Environmental cues such as shortening day length and declining temperature trigger endocrine and metabolic changes that slow development, reduce activity, and increase resistance to cold and dehydration. Diapause may occur during embryonic, larval, pupal, or adult stages and ends when internal timing mechanisms and favorable conditions permit development to resume. Studying winter diapause helps explain seasonal population dynamics, improve pest management, support conservation planning, and predict how species may respond to climate change.

Winter Diapause - Related Videos

Research

JoVE Journal - Biology

An Experimental and Bioinformatics Protocol for RNA-seq Analyses of Photoperiodic Diapause in the Asian Tiger Mosquito, Aedes albopictus

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Cited by 7 •

2014

RNA-Seq analyses are becoming increasingly important for identifying the molecular underpinnings of adaptive traits in non-model organisms. Here, a protocol to identify differentially expressed genes between diapause and non-diapause Aedes albopictus mosquitoes is described, from mosquito rearing, to RNA sequencing and bioinformatics analyses of RNA-Seq data.

Combining Histochemical Staining and Image Analysis to Quantify Starch in the Ovary Primordia of Sweet Cherry during Winter Dormancy

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Cited by 9 •

2019

We present a methodology to quantify the starch content in the ovary primordia in sweet cherry (Prunus avium L.) during winter dormancy by using an image analysis system combined with histochemical techniques.

Preparation of Complaint Matrices for Quantifying Cellular Contraction

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Cited by 115 •

2010

In this video, we demonstrate the experimental techniques used to fabricate compliant, extracellular matrix (ECM) coated substrates suitable for cell culture, and which are amenable to traction force microscopy and observing effects of ECM stiffness on cell behavior.

Visualization of Ambient Mass Spectrometry with the Use of Schlieren Photography

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Cited by 4 •

2016

This paper presents a protocol for the visualization of gaseous streams of an ambient ionization source using schlieren photography and mass spectrometry.

Education

JoVE Lab Manual - Biology

Natural Selection - Concepts

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2019

Fitness Widespread variation of phenotypes in natural populations provides the raw material for evolution, which is the change in the inherited traits of populations over successive generations. Natural selection is one of the main mechanisms of evolution and requires variable traits to be heritable and associated with differential survival and/or reproductive success. Phenotypes that correlate with greater success will have more offspring that survive to reproduce in the next generation, and...

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