Journal
/
Bioengineering
/
Preparation of Complaint Matrices for Quantifying Cellular Contraction
JoVE Journal
Bioengineering
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Bioengineering
Preparation of Complaint Matrices for Quantifying Cellular Contraction

Preparation of Complaint Matrices for Quantifying Cellular Contraction

DOI:
10.3791/2173-v

•

11:38 min

•

December 14, 2010

•

, , , ,
1Institute for Biophysical Dynamics,University of Chicago, 2Physics Department – James Franck Institute,University of Chicago, 3Interdisciplinary Scientist Training Program,University of Chicago

Chapters

  • 00:05Title
  • 01:15Coverslip Surface Activation
  • 03:00Polyacrylamide (PAA) Gel Preparation
  • 05:07Extracellular Matrix (ECM) Protein Crosslinking to the PAA Gel
  • 07:30Confocal Imaging
  • 09:47Representative Results for Quantifying Cellular Contraction
  • 11:15Conclusion

Summary

Automatic Translation

Automatic Translation

In this video, we demonstrate the experimental techniques used to fabricate compliant, extracellular matrix (ECM) coated substrates suitable for cell culture, and which are amenable to traction force microscopy and observing effects of ECM stiffness on cell behavior.

Tags

Complaint MatricesQuantifying Cellular ContractionCellular AdhesionExtracellular Matrix (ECM)Focal AdhesionsF-actin CytoskeletonMechanical ForcesCell MigrationECM RemodelingCell Culture SubstratesTraction Force Microscopy (TFM)Substrate DeformationsCytoskeletal OrganizationFluorescence Microscopy

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/2173?article_type=v

Models and Methods to Evaluate Transport of Drug Delivery Systems Across Cellular Barriers
check_url/2173?article_type=v

Preparation and Photoacoustic Analysis of Cellular Vehicles Containing Gold Nanorods
check_url/2173?article_type=v

Neonatal Cardiac Scaffolds: Novel Matrices for Regenerative Studies
check_url/2173?article_type=v

2D and 3D Matrices to Study Linear Invadosome Formation and Activity
check_url/2173?article_type=v

Automated Contraction Analysis of Human Engineered Heart Tissue for Cardiac Drug Safety Screening
check_url/2173?article_type=v

Mammalian Cell Division in 3D Matrices via Quantitative Confocal Reflection Microscopy
check_url/2173?article_type=v

Gel-seq: A Method for Simultaneous Sequencing Library Preparation of DNA and RNA Using Hydrogel Matrices
check_url/2173?article_type=v

Fabrication of a Multiplexed Artificial Cellular MicroEnvironment Array
check_url/2173?article_type=v

Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers
check_url/2173?article_type=v

Novel Process for 3D Printing Decellularized Matrices

Read Article