Cd3 Cd56 Phenotyping
Cd3 Cd56 Phenotyping - Related Videos
07:35
A Non-invasive Way to Isolate and Phenotype Cells from the Conjunctiva
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Cited by 5 •
2017
The exposed normal ocular surface consists of cornea and conjunctiva. Epithelial cells, goblet cells and immune cells are present in the conjunctiva. Here, a non-invasive, technique of impression cytology is described using an impression cytology device and flow cytometry to analyze immune cells in the conjunctiva.
07:17
Phenotypic and Functional Analysis of Activated Regulatory T Cells Isolated from Chronic Lymphocytic Choriomeningitis Virus-infected Mice
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Cited by 1 •
2016
Here, we describe a protocol to analyze the phenotype of regulatory T (Treg) cells isolated from naïve and chronic lymphocytic choriomeningitis virus-infected mice. In addition, we provide a process to evaluate the suppressive activity of the Treg cells.
08:49
Transduction and Expansion of Primary T Cells in Nine Days with Maintenance of Central Memory Phenotype
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Cited by 5 •
2020
We outline a 9-day protocol for the transduction and expansion of rhesus macaque peripheral blood mononuclear cells which yields cells with excellent co-expression of the genes of interest in sufficient number for infusion studies of cell efficacy.
05:35
CD Marker Recognition of Human Cytokine-Induced Killer Cells by Flow Cytometry
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2025
In this video, we demonstrate a flow cytometry-based technique to determine the proportion of cytokine-induced killer T-cells, CIK cells, expanded from a peripheral blood mononuclear cell culture.
09:40
RNAi Screening to Identify Postembryonic Phenotypes in C. elegans
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Cited by 9 •
2012
We describe a sensitized method to identify postembryonic regulators of protein expression and localization in C. elegans using an RNAi-based genomic screen and an integrated transgene that expresses a functional, fluorescently tagged protein.
FAQs
The interpretive value comes from evaluating the two markers together rather than using either one alone. CD3 positivity identifies a T-cell-associated population, CD56 positivity identifies a natural killer cell-associated population, and the combined pattern separates these groups within a mixed sample. This dual-marker strategy provides a more resolved view of lymphocyte composition.
Frequency indicates how much of each identified subset is present, whereas fluorescence intensity records the strength of the detected marker signal. Considering both measurements provides more information than reporting subset proportions alone. In cancer studies, these paired readouts can support comparisons of immune-cell composition across blood, tumor, and cultured samples.
Co-expression identifies a population that differs from cells classified only by CD3 or only by CD56. This group is described as NKT-like, allowing investigators to track it separately from CD3-positive T-cell and CD56-positive natural killer cell populations. Reporting the dual-positive frequency preserves an important feature of the overall immune profile.
A typical workflow applies fluorescently labeled antibodies to the mixed-cell sample and then analyzes the cells by flow cytometry. The detected signals are used to classify CD3-positive, CD56-positive, and dual-positive populations. Investigators can then report both the frequency and fluorescence intensity of these subsets for the sample under study.
The approach can be applied to blood, tumors, and cultured samples, enabling immune-cell composition to be examined in different experimental settings. Using the same CD3 and CD56 readouts across these sources supports comparisons of lymphocyte subsets and helps relate findings from tumor material to observations made in blood-based or culture-based studies.
In cancer research, the resulting subset profile supports immune-response studies, biomarker evaluation, and treatment-response investigations. Researchers can compare the frequencies and fluorescence intensities of CD3-positive, CD56-positive, and dual-positive populations to characterize immune changes. These measurements also contribute to investigations of immune-mediated tumor control and the cellular features associated with cancer treatment.