Cell Sonication
Cell Sonication - Related Videos
03:22
Low Frequency Ultrasound Application: A Method to Determine the Effect of Sonication on Human Leukemia Cells
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2023
This video describes the selective damage of human leukemia cells by using low frequency ultrasound. This application of low frequency ultrasound in a preclinical in vitro condition demonstrates that cell destruction can be attained in order to examine and compare the effects of sonication on leukemia and normal cells.
07:24
Sonication Extraction of Lipid Biomarkers from Sediment
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2023
Source: Laboratory of Jeff Salacup - University of Massachusetts Amherst The material comprising the living "organic" share of any ecosystem (leaves, fungi, bark, tissue; Figure 1) differs fundamentally from the material of the non-living "inorganic" share (rocks and their constituent minerals, oxygen, water, metals). Organic material contains carbon linked to a series of other carbon and hydrogen molecules (Figure 2), which distinguishes it from inorganic material. Carbon's wide valency range...
09:05
Generation and Quantitative Analysis of Pulsed Low Frequency Ultrasound to Determine the Sonic Sensitivity of Untreated and Treated Neoplastic Cells
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2015
Selective damage of human leukemia cells can be achieved through a novel approach of applying low frequency ultrasound both with and without chemotherapeutic pretreatment of leukemic and normal hematopoietic cells.
10:35
Novel Diagnostics in Revision Arthroplasty: Implant Sonication and Multiplex Polymerase Chain Reaction
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Cited by 16 •
2017
Differential diagnostics in painful arthroplasty is crucial for treatment success. Joint aspiration is routinely performed preoperatively. Multiplex polymerase chain reaction of the joint aspirate and the sonication fluid, a feasible tool for quick pathogen detection from these samples is described.
10:10
Sonication-facilitated Immunofluorescence Staining of Late-stage Embryonic and Larval Drosophila Tissues In Situ
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Cited by 5 •
2014
Immunostaining is an effective technique for visualizing specific cell types and proteins within tissues. By utilizing sonication, the protocol described here alleviates the need to dissect Drosophila melanogaster tissues from late-stage embryos and larvae before immunostaining. We provide an efficient methodology for the immunostaining of formaldehyde-fixed whole mount larvae.
FAQs
During Cell Sonication, acoustic energy creates microscopic bubbles in the liquid suspension. Their rapid formation and collapse produces localized shear forces that disrupt cell membranes, releasing intracellular contents without relying on a chemical lysis description. This mechanism makes proteins, nucleic acids, and other intracellular molecules accessible for downstream analysis or experimental preparation.
The extent of disruption depends on applied power, pulse duration, temperature, and sample volume. Changing these conditions alters how much acoustic energy reaches the suspension, so identical samples can yield different lysates if settings vary. Controlling these variables is therefore central to reproducible cell lysis and consistent molecular measurements.
Temperature deserves separate attention because sonication can damage heat-sensitive intracellular targets. Monitoring and controlling sample temperature helps balance sufficient membrane disruption against unwanted heat-related effects. In cancer research, this consideration is important when preparing lysates for protein or nucleic-acid analysis, where target integrity can influence the quality and interpretability of downstream results.
A practical workflow places tumor or cultured-cell material in a liquid suspension, applies acoustic energy with an ultrasonic probe or bath, and adjusts power, pulse duration, temperature, and sample volume. The resulting disrupted material can then serve as a lysate or homogenized preparation for downstream assays and molecular analyses.
Sample preparation requires a liquid suspension and an acoustic-energy device, either an ultrasonic probe or an ultrasonic bath. The relevant setup also includes control of power, pulse duration, temperature, and sample volume. These elements determine how disruption is applied and provide the main variables for adapting the procedure to a particular sample.
Cell Sonication supports several cancer-research workflows: lysing cultured cells or tumor samples, homogenizing those materials, extracting proteins and nucleic acids, and preparing lysates for Western blotting or molecular profiling. Its value lies in converting cellular material into preparations compatible with these analyses, allowing intracellular molecular content to be examined in a structured experimental workflow.
Optimization balances two outcomes: adequate cell disruption and preservation of sensitive intracellular targets. Researchers can adjust power, pulse duration, temperature, and sample volume rather than assuming one setting fits every preparation. This balance improves reproducibility between samples while reducing the risk that heat-related damage will compromise subsequent protein or nucleic-acid analyses.