Icg Imaging
Icg Imaging - Related Videos
09:36
The Assessment of Lymphatic Drainage Using ICG Near-Infrared Imaging and Ultrasound After Fracture
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2026
This protocol assesses lymphatic drainage after fracture by combining indocyanine green(ICG) near-infrared imaging with ultrasound to quantify plantar ICG clearance and popliteal lymph node enlargement in mice.
07:42
Labeling Stem Cells with Fluorescent Dyes for non-invasive Detection with Optical Imaging
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Cited by 33 •
2008
This video shows techniques for labeling of human embryonic stem cells and mesenchymal stem cells with fluorescent dyes. This technique can be used for an in vivo tracking of transplanted stem cells with optical imaging and for histopathological correlations with fluorescence microscopy.
09:52
Non-invasive Optical Imaging of the Lymphatic Vasculature of a Mouse
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Cited by 25 •
2013
Recently developed imaging techniques using near-infrared fluorescence (NIRF) may help elucidate the role the lymphatic system plays in cancer metastasis, immune response, wound repair, and other lymphatic-associated diseases.
06:37
Multispectral Real-time Fluorescence Imaging for Intraoperative Detection of the Sentinel Lymph Node in Gynecologic Oncology
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Cited by 41 •
2010
Fluorescence imaging is a promising innovative modality for image-guided surgery in surgical oncology. In this video we describe the technical procedure for detection of the sentinel lymph node using fluorescence imaging as showcased in gynecologic oncologicy. A multispectral fluorescence camera system, together with the fluorescent agent indocyanine green, is applied.
13:45
Hybrid µCT-FMT imaging and image analysis
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Cited by 34 •
2015
We describe a protocol for hybrid imaging, combining fluorescence-mediated tomography (FMT) with micro computed tomography (µCT). After fusion and reconstruction, we perform interactive organ segmentation to extract quantitative measurements of the fluorescence distribution.
FAQs
Once administered, ICG binds to plasma proteins, creating the fluorescent form detected by the imaging system. Illumination causes the bound dye to emit near-infrared light, while the camera captures the resulting signal. This interaction connects dye distribution in the circulation with visible contrast, allowing investigators to examine vascular patterns and tissue-associated fluorescence during cancer studies.
Signal visibility depends strongly on how deeply the target lies beneath the tissue surface and where the dye distributes after administration. Near-infrared fluorescence can be detected through limited tissue depths, so superficial or favorably distributed signals may appear clearer than deeper ones. These constraints must be considered when interpreting tumor localization, perfusion, or lymphatic findings.
Near-infrared detection provides a fluorescence signal that a dedicated camera system can monitor during an imaging procedure. Because the contrast is observed as the procedure occurs, investigators can relate tissue appearance to the immediate surgical or experimental setting. This supports dynamic assessment rather than relying only on findings examined after the procedure has ended.
Fluorescence patterns can provide information about where selected tumors are located and how nearby blood flow or vascular perfusion behaves. In cancer research, these observations help connect tumor position with circulation-related features. The method therefore contributes not only to finding tissue, but also to studying biological differences that may influence image-guided investigation and intervention.
A typical workflow administers the injectable dye, illuminates the relevant tissue with the imaging system, and records the emitted near-infrared fluorescence. Investigators then assess the observed signal in relation to tissue location, blood flow, lymphatic pathways, or perfusion. The sequence supports immediate visualization, while dye distribution and limited penetration guide interpretation of the images.
The technique is particularly useful when researchers need visual guidance while locating a tumor, tracing lymphatic pathways, or examining vascular perfusion. Its real-time contrast can help align the observed fluorescence with the operative or experimental field. These applications make it relevant to image-guided procedures in which tissue position and circulation must be assessed during the intervention.
After dye administration, the fluorescence pattern can be examined to identify pathways or regions associated with lymphatic drainage and to help locate selected tumors. The camera displays these signals during the procedure, giving investigators immediate spatial information. This can support cancer research protocols focused on mapping tissue relationships or improving the precision of image-guided surgical work.
Images should be interpreted with attention to signal depth and dye distribution. Fluorescence may be difficult to detect when a target lies beyond the technique’s limited tissue penetration, and uneven distribution can affect the apparent location or intensity of a signal. Consequently, image quality and biological interpretation depend on both the optical constraints and the dye’s movement through tissues.