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JoVE Journal
Immunology and Infection

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Phagosome Migration and Velocity Measured in Live Primary Human Macrophages Infected with HIV-1
 

Phagosome Migration and Velocity Measured in Live Primary Human Macrophages Infected with HIV-1

Article DOI: 10.3791/54568
September 5th, 2016

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Summary September 5th, 2016

We describe a method to measure the velocity of phagosomes moving towards the cell center in living cells infected with or without the human immunodeficiency virus (HIV) type 1, using spinning disk confocal fluorescence microscopy to identify fluorescent infected cells and bright field microscopy to detect phagosomes.

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