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Immunology and Infection

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JoVE Journal Immunology and Infection
Phagosome Migration and Velocity Measured in Live Primary Human Macrophages Infected with HIV-1
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Phagosome Migration and Velocity Measured in Live Primary Human Macrophages Infected with HIV-1

Article DOI: 10.3791/54568-v • 07:35 min • September 5th, 2016
September 5th, 2016 •
1, 1, 1, 1
1Inserm U1016, Institut Cochin, CNRS UMR 8104, Université Paris Descartes, Sorbonne Paris Cité

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Summary
Automatic Translation

We describe a method to measure the velocity of phagosomes moving towards the cell center in living cells infected with or without the human immunodeficiency virus (HIV) type 1, using spinning disk confocal fluorescence microscopy to identify fluorescent infected cells and bright field microscopy to detect phagosomes.

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Phagosome Migration Phagosome Velocity Live Primary Human Macrophages HIV-1 Infection Manual Tracking Method Relative Movements Intercellular Compartments Fluorescently Tagged Cells Phagocytosis Assay Confocal Imaging System Spinning Disc Microscope Heating Chamber Imaging Software Scanning Speed Magnification Resolution
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