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Immunology and Infection
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Phagosome Migration and Velocity Measured in Live Primary Human Macrophages Infected with HIV-1
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Immunology and Infection
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JoVE Journal Immunology and Infection
Phagosome Migration and Velocity Measured in Live Primary Human Macrophages Infected with HIV-1

Phagosome Migration and Velocity Measured in Live Primary Human Macrophages Infected with HIV-1

DOI:
10.3791/54568-v

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07:32 min

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September 05, 2016

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, , ,
1Inserm U1016, Institut Cochin, CNRS UMR 8104, Université Paris Descartes, Sorbonne Paris Cité

Chapters

  • 00:05Title
  • 00:49Live Cell Video Microscopy Phagocytosis Assay
  • 02:41Analysis of Time Lapse Movies
  • 05:29Results: Velocity of Phagosome Migration in HIV-infected and Uninfected Human Macrophages
  • 06:20Conclusion

Summary

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We describe a method to measure the velocity of phagosomes moving towards the cell center in living cells infected with or without the human immunodeficiency virus (HIV) type 1, using spinning disk confocal fluorescence microscopy to identify fluorescent infected cells and bright field microscopy to detect phagosomes.

Tags

Keywords: Phagosome MigrationPhagosome VelocityHIV-1 Infected MacrophagesLive Cell ImagingConfocal MicroscopyPhagocytosis AssaySheep-bred Blood CellsGFP-positiveTime-lapse VideoVideo Editing SoftwareManual Tracking Method

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