Micropipette Transfer
Micropipette Transfer - Related Videos
10:31
Micropipette Aspiration of Substrate-attached Cells to Estimate Cell Stiffness
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Cited by 30 •
2012
Here we describe a quick and simple method to measure cell stiffness. The general principle of this approach is to measure membrane deformation in response to well-defined negative pressure applied through a micropipette to the cell surface. This method provides a powerful tool to study biomechanical properties of substrate-attached cells.
02:43
Fly Aspirator Tube: A Simple Tool to Transfer Live Drosophila
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2023
This video describes one method to build a fly aspirator, which can be used to transfer insects between enclosures without anesthesia. The aspirator in the example protocol was prepared for an experiment to quantify individual grooming...
01:20
Transfer Pricing
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2026
Transfer pricing is the process of setting prices for goods, services, or intangible assets exchanged between divisions, subsidiaries, or related entities within the same organization. Although these transactions occur within a single company, transfer prices are important for evaluating divisional performance, allocating resources, and supporting managerial decision-making. They affect the reported revenues, costs, and profitability of individual business units.Organizations use several...
07:02
Fluorescence Micropipette Aspiration Assay to Investigate Red Blood Cell Mechanosensing
0 Views •
Cited by 1 •
2024
The exploration of cellular behavior under mechanical stress is pivotal for advances in cellular mechanics and mechanobiology. We introduce the Fluorescence Micropipette Aspiration (fMPA) technique, a novel method combining controlled mechanical stimulation with comprehensive analysis of intracellular signaling in single cells. This technique investigates new in-depth studies of live-cell mechanobiology.
02:59
Agarose-Embedded Electroporation-Assisted Gene Transfer in Mouse Cortical Interneuron Progenitors
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2025
Source: Denaxa, M., el al., Transplantation of Chemogenetically Engineered Cortical Interneuron Progenitors into Early Postnatal Mouse Brains. J. Vis. Exp. (2019)This video demonstrates the procedure for injecting a plasmid vector carrying the gene of interest into interneuron progenitors in an embryonic brain slice, followed by electroporation for plasmid entry and gene expression. These modified progenitors can later be used for treating brain disorders.
FAQs
The plunger stops regulate how liquid enters and leaves the tip. Releasing the plunger after pressing it creates the pressure difference that draws in the selected volume. During dispensing, pressing to the first stop releases the measured liquid, while pressing to the second stop helps expel the remaining contents, supporting consistent transfer between samples.
Tip position and handling affect whether the selected volume enters the tip cleanly. Appropriate immersion allows liquid aspiration without disrupting the transfer, while steady movement helps limit bubbles. These practices also reduce opportunities for contamination, which is important when small volume differences or sample carryover could affect reproducibility in cancer research experiments.
Calibration links the pipette setting to the volume actually transferred, making it central to accurate and reproducible work. Without reliable calibration, dilution steps, reagent distribution, and sample preparation may vary between transfers. Consistent volume delivery therefore helps researchers compare results across cancer research assays and maintain dependable preparation of biological materials.
A typical transfer uses a disposable tip and a calibrated volume setting. The operator presses and releases the plunger to aspirate liquid after positioning the tip appropriately, then dispenses the sample by pressing through the first stop to the second stop. Steady handling throughout the aspiration and dispensing sequence helps limit bubbles and contamination.
Researchers use the technique to distribute assay reagents and dilute samples in a controlled, repeatable manner. Consistent handling helps ensure that related assay preparations receive comparable liquid volumes, which supports meaningful comparison of experimental conditions. This role is especially relevant to cancer research workflows involving diagnostics and drug screening, where preparation quality influences experimental consistency.
Micropipette transfer supports preparation of cell cultures and loading of nucleic acid or protein preparations. Accurate handling helps researchers place the intended materials into the appropriate experimental preparations without introducing unnecessary variation from inconsistent volumes. In cancer research, these transfers contribute to molecular analysis as well as broader workflows that depend on reliable sample and reagent preparation.