Orthotopic Tumor Growth
Orthotopic Tumor Growth - Related Videos
06:23
A Murine Orthotopic Bladder Tumor Model and Tumor Detection System
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Cited by 8 •
2017
This protocol describes the generation of murine orthotopic bladder tumors in female C57BL/6J mice and the monitoring of tumor growth.
03:59
Orthotopic Homograft Tumor Transplantation: A Technique to Generate Pancreatic Tumor Mouse Models
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2023
The video explains a stepwise surgical method for orthotopic implantation of a tumor fragment from a cancerous donor to the pancreas of a non-cancerous recipient mice for development of pancreatic cancer. Orthotopically implanted PDAC could recapture the tissue microenvironment of human pancreatic cancers than conventional subcutaneous (SC) models more accurately.
04:11
DCE-MRI of Orthotopic Pancreatic Tumor Xenografts: A Method to Assess Microvasculature in a Target Tumor Tissue
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2023
This video describes the technique of DCE-MRI for orthotropic pancreatic tumor xenografts in mice. DCE-MRI is a non-invasive method to analyze microvasculature in a target tissue, and useful to assess vascular response in a tumor following a novel therapy. This method will assist investigators to apply DCE-MRI for orthotopic gastrointestinal cancer mouse models.
09:43
Primary Orthotopic Glioma Xenografts Recapitulate Infiltrative Growth and Isocitrate Dehydrogenase I Mutation
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Cited by 10 •
2014
Malignant gliomas constitute a heterogeneous group of highly infiltrative glial neoplasms with distinct clinical and molecular features. Primary orthotopic xenografts recapitulate the histopathological and molecular features of malignant glioma subtypes in preclinical animal models.
10:35
An Orthotopic Model of Serous Ovarian Cancer in Immunocompetent Mice for in vivo Tumor Imaging and Monitoring of Tumor Immune Responses
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Cited by 18 •
2010
To study in vivo tumor growth and tumor microenvironment, we used a syngeneic and orthotopic mouse model of ovarian cancer in immunocompetent animals. We transduced a mouse tumor cell line (MOV1) with Katushka fluorescent protein (MOV1KAT) and here we show its orthotopic implantation in ovary and in vivo imaging.
FAQs
The local microenvironment shapes tumor progression through interactions among the implanted cancer material, surrounding tissue, blood vessels, stromal cells, and immune components. These site-specific relationships can influence how the tumor develops within its native organ context. Consequently, the model can reveal biological behavior that may be less apparent when tumor material grows outside the corresponding anatomical site.
An orthotopic model preserves the relationship between tumor material and the tissue where that cancer naturally originates, whereas an ectopic model places it elsewhere. This anatomical distinction can affect tissue-specific invasion, metastatic behavior, and responses to treatment. Comparing the two approaches helps researchers determine how strongly local tissue context contributes to observed cancer phenotypes and therapeutic outcomes.
Researchers may establish the model with tumor cells, tissue fragments, or patient-derived material. These starting materials provide different ways to study tumor development while retaining interactions with the matching tissue environment. Selecting among them allows cancer studies to focus on questions involving tumor biology, treatment response, drug delivery, imaging, or patient-specific therapeutic strategies, when appropriate material is available.
A typical workflow begins by selecting tumor cells, a tissue fragment, or patient-derived material that corresponds to the cancer of interest. The material is then implanted into the matching anatomical site so it can develop within the relevant organ environment. Researchers subsequently examine tumor progression and related outcomes, such as invasion, metastasis, imaging features, or treatment response.
This approach is useful when treatment performance may depend on the tumor’s native tissue environment. Because the model incorporates local blood vessels, stromal cells, immune components, and surrounding tissue, it can support evaluation of therapeutic efficacy and drug delivery. Researchers can also use it to examine whether treatment responses observed in a relevant anatomical setting differ from those in ectopic systems.
Patient-derived material can be implanted into the corresponding anatomical site to create a model connected to an individual cancer source. Such models support investigation of tumor progression, treatment responses, and drug delivery in a tissue-relevant setting. In cancer research, this makes orthotopic systems useful for exploring personalized treatment strategies alongside broader studies of tumor biology and therapeutic efficacy.