Pediatric Cancer Imaging
Pediatric Cancer Imaging - Related Videos
15:18
Making MR Imaging Child's Play - Pediatric Neuroimaging Protocol, Guidelines and Procedure
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Cited by 139 •
2009
Despite an increase in the use of structural and functional magnetic resonance imaging (fMRI) in humans, the study of young pediatric populations remains a challenge. We present a hands-on, step-by-step video protocol including guidelines for clinicians and researchers intending to perform (f)MRI in young children.
03:01
Immunocytochemistry-Based Imaging for Visualizing Pediatric Glioma Cell Migration
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2025
Source: Pericoli, G. et. al., Live-3D-Cell Immunocytochemistry Assays of Pediatric Diffuse Midline Glioma. J. Vis. Exp. (2021)This video demonstrates live cell imaging to observe pediatric diffuse midline glioma cell migration using immunohistochemical labeling with anti-CD44 antibodies.
04:27
Co-culturing Glutamatergic Neurons and Pediatric High-Grade Glioma Cells
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2025
This video showcases a technique for co-culturing glutamatergic neurons and pediatric high-grade glioma (pHGG) cells within a microfluidic device. Human induced pluripotent stem cells are introduced into the microfluidic system and incubated to promote their differentiation into mature cortical glutamatergic neurons. Following this, the pHGG cells are introduced onto the mature neurons, establishing a co-culture and enabling interactions between neurons and pHGG cells.
08:45
Cerenkov Luminescence Imaging (CLI) for Cancer Therapy Monitoring
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Cited by 8 •
2012
Use of Cerenkov Luminescence Imaging (CLI) for monitoring preclinical cancer treatment is described here. This method takes advantage of Cerenkov Radiation (CR) and optical imaging (OI) to visualize radiolabeled probes and thus provides an alternative to PET in preclinical therapeutic monitoring and drug screening.
09:43
Transplantation of Zebrafish Pediatric Brain Tumors into Immune-competent Hosts for Long-term Study of Tumor Cell Behavior and Drug Response
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Cited by 22 •
2017
The transplantation of cancer cells is an important tool for the identification of cancer mechanisms and therapeutic responses. Current techniques depend on immune-incompetent animals. Here, we describe a method to transplant zebrafish tumor cells into immune-competent embryos for the long-term analysis of tumor cell behavior and in vivo drug responses.
FAQs
Selecting an imaging modality requires balancing the information needed with the child’s developing anatomy and potential long-term safety concerns. Ultrasound and MRI provide approaches that do not use x-rays, while CT uses x-rays and PET uses radiotracers. Tailored protocols help limit unnecessary radiation exposure without losing information needed for diagnosis, staging, treatment planning, or follow-up.
Anatomical imaging shows the location and physical features of suspected disease, whereas functional imaging provides information about biological activity. MRI, CT, and ultrasound can contribute anatomical detail through different physical signals, while PET uses radiotracers to generate functional information. Combining these perspectives can improve tumor characterization and help evaluate disease beyond what one imaging approach shows alone.
Each modality answers different clinical questions, so combining them can provide a more complete assessment. Ultrasound may contribute information using sound waves, MRI uses magnetic fields and radiofrequency signals, CT uses x-rays, and PET uses radiotracers. Their complementary anatomical and functional information can support evaluation of the primary tumor, possible spread, and changes during treatment.
The workflow begins by identifying whether the main need is detection, characterization, staging, treatment planning, biopsy guidance, or response assessment. Clinicians then select an appropriate imaging approach based on the information required and the need to limit unnecessary radiation exposure. Using a question-driven strategy makes the resulting images more relevant to decisions in diagnosis and care.
Imaging can show where a tumor is located and how it relates to the surrounding anatomy, which helps guide biopsy or plan treatment. Characterization and staging add information about tumor features and possible spread before intervention. This visual guidance supports more informed decisions by connecting the suspected disease with the specific location and extent seen on imaging.
Imaging performed over time can provide a basis for comparing disease status during monitoring. Repeated assessments may show whether findings have changed after treatment, while anatomical or functional information can contribute different evidence about response. In cancer research and clinical care, this longitudinal information supports evaluation of outcomes and helps inform subsequent decisions about management.