Phase-bright Cells
Phase-bright Cells - Related Videos
10:11
Cell Co-culture Patterning Using Aqueous Two-phase Systems
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Cited by 32 •
2013
Aqueous two-phase systems were used to simultaneously pattern multiple populations of cells. This fast and easy method for cell patterning takes advantage of the phase separation of aqueous solutions of dextran and polyethylene glycol and the interfacial tension that exists between the two polymer solutions.
02:39
Phase Diagrams
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2020
A phase diagram combines plots of pressure versus temperature for the liquid-gas, solid-liquid, and solid-gas phase-transition equilibria of a substance. These diagrams indicate the physical states that exist under specific conditions of pressure and temperature and also provide the pressure dependence of the phase-transition temperatures (melting points, sublimation points, boiling points). Regions or areas labeled solid, liquid, and gas represent single phases, while lines or curves represent...
03:24
BrdU Immunofluorescence Staining: A Technique to Identify Cells in Different Phases of Cell Cycle
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2023
This video describes the protocol for BrdU immunofluorescence staining. The technique allows the identification of cells in different phases of cell cycle. Addition of DNA dyes and antibody labeling allows the detailed analysis of the fate of the S phase cells at later times.
09:14
Preparing a Celadonite Electron Source and Estimating Its Brightness
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2019
The article presents a protocol to prepare a celadonite source and estimate its brightness for use in a long-range imaging low-energy electron point-source projection microscope.
02:31
Phase Transitions
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2020
Whether solid, liquid, or gas, a substance's state depends on the order and arrangement of its particles (atoms, molecules, or ions). Particles in the solid pack closely together, generally in a pattern. The particles vibrate about their fixed positions but do not move or squeeze past their neighbors. In liquids, although the particles are closely spaced, they are randomly arranged. The position of the particles are not fixed—that is, they are free to move past their neighbors to occupy...
FAQs
Brightness arises when cell features alter the phase of transmitted light in a way that the microscope renders as intensity contrast. Greater thickness or a different refractive index changes how light passes through the cell, while rounding, condensation, or detachment can accentuate the appearance. Thus, phase brightness reflects optical and morphological differences rather than a fluorescent signal.
Cell shape and physical state strongly influence the signal. A cell that rounds up, becomes condensed, or loses attachment may look brighter than a spread, attached cell, even without a change in fluorescent labeling because no label is required. Interpreting brightness therefore works best alongside visible morphology, since the appearance can reflect several related structural changes.
Compared with fluorescence-based observation, phase-contrast viewing supplies visual information without fluorescent labels. Its contrast comes from converting phase changes in transmitted light into intensity differences, so researchers can examine morphology and culture behavior directly. The approach is especially useful when the question concerns attachment, confluence, growth, or visible changes during treatment rather than a labeled molecular signal.
In cancer cultures, an increase in conspicuously bright cells can coincide with rounding, condensation, detachment, or other visible changes associated with proliferation, mitosis, stress, or loss of viability. The observation provides a morphological cue for deciding which cellular states warrant closer assessment, but brightness alone does not identify one biological process conclusively.
Examine individual-cell appearance together with attachment, overall confluence, and growth as the culture changes. Recording these features during culture helps distinguish a localized shift in cell morphology from a broader change in the population. This combined view is more informative than counting bright cells alone because it places the optical appearance within the culture’s structural and growth context.
Researchers can compare the appearance and frequency of phase-bright cells before and after treatment while also noting changes in morphology, attachment, confluence, and growth. Rounding, condensation, or detachment may signal a treatment-associated cellular change, including stress or reduced viability. These observations support response assessment by revealing visible effects in cultured cells without requiring fluorescent labeling.