Segmental Colon Infection
Segmental Colon Infection - Related Videos
10:32
In Vitro Recording of Mesenteric Afferent Nerve Activity in Mouse Jejunal and Colonic Segments
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Cited by 8 •
2016
Mesenteric afferent nerves convey information from the gastrointestinal tract towards the brain regarding normal homeostasis as well as pathophysiology. Gastrointestinal afferent nerve activity can be assessed by mounting isolated intestinal segments with attached afferent nerves into an organ bath, isolating the nerve, and assessing basal as well as stimulated activity.
05:19
Segmental Adeno-Cre Infection: A Technique to Generate Isolated Colorectal Cancer using Genetically Engineered Mouse Models
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2023
This video describes the generation of a colorectal mouse model by segmental adeno-cre infection. The infected colon segment leads to tumorigenesis within this segment of the mucosa, resulting in adenomas ultimately progressing to invasive and metastatic carcinoma. This model is an attractive platform for studies of cancer biology and preclinical therapeutic trials.
03:20
Electrophysiological Recording from Colonic Afferent Nerves in an Ex Vivo Rat Colon
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2025
This video demonstrates a method to record the activity of colonic afferent nerves in an ex-vivo rat colon, cannulated at both ends and infused with saline to increase intraluminal pressure. As pressure increases, the nerve signal rises, indicating its stimulation.
02:18
Assessing Bacterial Colonization in a Mouse Brain following Listeria monocytogenes Infection
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2025
This video demonstrates the quantification of the infective bacterial load from the perfused mouse brain infected with Listeria monocytogenes.
09:18
Assessing Bacterial Invasion of Cardiac Cells in Culture and Heart Colonization in Infected Mice Using Listeria monocytogenes
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Cited by 4 •
2015
Listeria monocytogenes causes fetal infections in pregnant women and meningitis in susceptible populations. Subpopulations of bacteria can colonize cardiac tissue, causing myocarditis in patients and laboratory animals. Here we present a protocol that describes how to assess L. monocytogenes cardiac cell invasion in vitro and cardiac colonization in infected animals.
FAQs
Microbial invasion or toxin activity can compromise the intestinal epithelial barrier, exposing underlying tissue to additional microbial products and damage signals. This disruption activates innate immune signaling and promotes recruitment of immune cells within the affected region. In cancer research, linking barrier injury to local inflammation helps investigators study how infection-associated tissue damage may create conditions relevant to colorectal tumor development.
The defined affected segment allows researchers to compare injured, inflamed tissue with neighboring areas that remain less involved. This spatial contrast can help distinguish responses associated with infection from features of the surrounding intestinal environment. Such comparisons are useful for examining regional epithelial injury, localized immune activity, and the extent to which inflammation remains confined or influences nearby tissue.
The model connects microbial activity and inflammatory tissue responses with processes relevant to colorectal cancer. Investigators can examine whether epithelial injury, innate immune activation, and recruited immune cells alter the local environment in ways associated with tumor initiation or progression. This provides a framework for studying infection and inflammation as interacting influences rather than analyzing tumor biology in isolation.
Regional epithelial injury, activation of innate immune signaling, and immune-cell recruitment provide complementary indicators of the host response. Examining these features together can show how microbial invasion or toxin activity affects the mucosa and surrounding tissue. In cancer-focused studies, their relationship may help identify inflammatory conditions that accompany infection-associated damage and altered tissue environments.
Researchers can evaluate host–microbe interactions, the distribution of mucosal injury, and inflammatory changes within the affected colon segment. The model also supports investigation of biomarkers associated with epithelial damage. Because neighboring tissue may be less involved, measurements can be interpreted against a regional tissue context, helping clarify which signals are linked specifically to the infected area.
In cancer research, the model can be used to examine how infection and inflammation modify the tissue environment relevant to colorectal tumor biology. Studies may focus on links between microbial activity, barrier disruption, immune recruitment, and tumor initiation or progression. This application broadens cancer analysis by incorporating local host–microbe interactions and inflammation-associated changes in the colon.
The system can support studies of treatments intended to limit infection-associated epithelial injury and inflammation while preserving normal intestinal function. Researchers can assess whether an intervention reduces regional mucosal damage or inflammatory responses without broadly disrupting unaffected tissue. This balance is important when treatment aims to control harmful consequences of infection while maintaining the colon’s normal environment.