Chromatogram Analysis
Chromatogram Analysis - Related Videos
11:02
Fruit Volatile Analysis Using an Electronic Nose
0 Views •
Cited by 10 •
2012
A rapid method for volatile compound analysis in fruit is described. The volatile compounds present in the headspace of a homogenate of the sample are rapidly separated and detected with ultra-fast gas chromatography (GC) coupled with a surface acoustic wave (SAW) sensor. A procedure for data handling and analysis is also discussed.
08:11
Quantitative Analysis of Chromatin Proteomes in Disease
0 Views •
Cited by 4 •
2012
Advances in mass spectrometry have allowed the high throughput analysis of protein expression and modification in a host of tissues. Combined with subcellular fractionation and disease models, quantitative mass spectrometry and bioinformatics can reveal new properties in biological systems. The method described herein analyzes chromatin-associated proteins in the setting of heart disease and is readily applicable to other in vivo models of human disease.
10:41
Carbon and Nitrogen Analysis of Environmental Samples
0 Views •
2023
Source: Laboratories of Margaret Workman and Kimberly Frye - Depaul University Elemental Analysis is a method used to determine elemental composition of a material. In environmental samples such as soils, scientists are particularly interested in the amounts of two ecologically important elements, nitrogen and carbon. Elemental analysis by the flash combustion technique works by oxidizing the sample with a catalyst through combustion in a high-temperature chamber. The products of combustion are...
11:36
Glycan Node Analysis: A Bottom-up Approach to Glycomics
0 Views •
Cited by 16 •
2016
This article presents an enhanced form of a novel bottom-up glycomics technique designed to analyze the pooled compositional profile of glycans in unfractionated biofluids through the chemical breakdown of glycans into their constituent linkage-specific monosaccharides for detection by GC-MS. Potential applications include early detection of cancer and other glycan-affective disorders.
11:02
Complete Workflow for Analysis of Histone Post-translational Modifications Using Bottom-up Mass Spectrometry: From Histone Extraction to Data Analysis
0 Views •
Cited by 227 •
2016
This protocol outlines a fully integrated workflow for characterizing histone post-translational modifications using mass spectrometry (MS). The workflow includes histone purification from cell cultures or tissues, histone derivatization and digestion, MS analysis using nano-flow liquid chromatography and instructions for data analysis. The protocol is designed for completion within 2 - 3 days.
FAQs
Retention time or elution volume provides a comparison coordinate, not an identity by itself. Analysts compare a sample peak's position with a standard analyzed under the relevant chromatographic conditions. Agreement supports assigning the component, while the full interpretation also considers whether the signal is distinct from baseline noise.
Peak area generally captures the detector response across the elution event, whereas peak height reflects the maximum response at one point. After calibration with standards, either measurement can support concentration estimates, but the selected metric must be applied consistently. This makes calibration and comparable integration settings essential when samples are compared.
Baseline correction removes the underlying signal used to separate analyte response from background, and integration then assigns a numerical response to the selected peak. If the baseline is placed inconsistently, calculated areas can change even when the sample does not. Careful treatment therefore improves comparisons among chromatograms and supports more reliable quantification.
Changes in peak positions or responses can indicate that sample composition differs, while the presence, absence, or relative size of peaks helps characterize the mixture. Interpretation should use standards and calibrated measurements rather than visual appearance alone. This approach is useful when assessing whether a reaction progresses, a material remains pure, or samples differ.
An effective workflow begins by reviewing detector response against retention time or volume, correcting the baseline, selecting relevant peaks, and integrating them. Analysts then compare peak positions with standards for identification and use calibrated peak areas or heights for concentration. Recording the same processing choices across samples makes comparisons more consistent.
Reaction monitoring uses changes in chromatographic signals to follow sample composition, while purity testing examines whether additional components appear alongside the expected material. Because peak positions support component assignment and calibrated responses support measurement, the same output can provide both qualitative and quantitative evidence. These uses help characterize chemical mixtures during laboratory work.
In pharmaceutical quality control, the analysis can support checks of composition and purity. Environmental measurement uses the same interpretive principles to assess components in samples, while sample characterization applies them to describe mixture composition. Gas chromatography and high-performance liquid chromatography can both produce chromatograms for this kind of chemical assessment.