Drop Counter System
Drop Counter System - Related Videos
09:33
Determining Cell Number During Cell Culture using the Scepter Cell Counter
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Cited by 56 •
2010
The Scepter Cell Counter is a handheld automated device that can be used to count cells, monitor cell diameter and volume, and be used to check the health and quality of cellular populations from one culture to the next.
09:20
Picoinjection of Microfluidic Drops Without Metal Electrodes
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Cited by 2 •
2014
We have developed a technique for picoinjecting microfluidic drops that does not require metal electrodes. As such, devices incorporating our technique are simpler to fabricate and to use.
07:24
A Simple Hanging Drop Cell Culture Protocol for Generation of 3D Spheroids
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Cited by 516 •
2011
We describe a simple, rapid method of generating 3D tissue-like spheroids and their potential application to quantify differences in cell-cell interactions.
01:13
Excess Pressure Inside a Drop and a Bubble
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2023
The shape of a small drop of liquid can be considered spherical, neglecting the effect of gravity. This drop can further be considered as two equal hemispherical drops put together due to surface tension. The forces acting on the spherical drop are due to the pressure of the liquid inside the drop, the pressure due to air outside the drop, and the force due to the surface tension acting on the two hemispherical drops. where γ corresponds to the surface tension. Recalling that the force per...
08:45
Glass-Based Devices to Generate Drops and Emulsions
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Cited by 1 •
2022
Here, a protocol to manufacture glass-based microfluidic devices used for generating highly monodisperse emulsions with controlled drop size is presented.
FAQs
As each liquid drop passes through the sensing region, it interrupts an optical or infrared light beam. That interruption produces an electrical pulse, and the system counts the pulses as individual delivery events. The resulting count provides a reproducible record of addition activity, allowing researchers to monitor dispensing without relying on manual visual counting.
A drop count does not directly represent a universal volume because the system must relate counted drops to the liquid being delivered. Calibration establishes the relationship between the number of detected drops and the estimated volume. This conversion makes the recorded data useful for controlled reagent addition, titrations, and quantitative comparisons between experimental runs.
The light-beam interruption provides a distinct event for each detected drop, which reduces dependence on an operator observing and counting drops manually. Each event becomes an electrical pulse that can be recorded consistently. In chemistry experiments, this supports more reproducible delivery records and helps reduce counting errors that could otherwise affect reagent quantities or comparisons.
The system is arranged so that delivered drops pass through the optical or infrared sensing region, where interruptions can be detected and recorded. Before using the readings as volume data, the drop count is calibrated against the estimated delivered volume. Once configured, the recorded pulses can be associated with reagent addition, titration progress, or flow measurements.
During titration or other reagent additions, the system records the number of delivered drops rather than requiring continuous manual counting. After calibration, those counts provide an estimated volume and a more consistent addition record. This can improve procedural reproducibility, support controlled dosing, and reduce errors caused by differences in how operators count or deliver drops.
Drop counts can be correlated with changes occurring during a chemical reaction, allowing delivered reagent amounts to be considered alongside reaction behavior. The system therefore supports more than dispensing control: it can contribute to quantitative analysis by connecting addition history with observed experimental changes. This is relevant in both teaching laboratories and research settings.