Grafting Complex
Grafting Complex - Related Videos
07:37
Mouse Models for Graft Arteriosclerosis
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Cited by 17 •
2013
We describe protocols for our mouse graft arteriosclerois (GA) models which involve interposition of a mouse vessel segment into a recipient of the same inbred strain. By backcrossing additional genetic changes into the vessel donor, the model can assess the effect of specific genes on GA.
03:35
A Technique for Human Skin Grafting in a Mouse Model
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2025
This video demonstrates a method for grafting human skin onto an immunodeficient mouse with a suppressed neutrophil response. The method involves applying human split-thickness skin to the mouse's graft bed, sealing it, and covering it for recovery. Eventually, the human skin adheres to the mouse's skin, establishing vascularization and circulation, resulting in a successful skin xenograft.
05:47
Isolation and Transplantation of Different Aged Murine Thymic Grafts.
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Cited by 14 •
2015
This report provides a detailed description of transplanting murine thymi from different aged donor mice under the kidney capsule of immunodeficient mouse recipients. The goal of this approach is to model T cell development and thymic selection events in vivo.
13:04
Generation and Grafting of Tissue-engineered Vessels in a Mouse Model
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Cited by 13 •
2015
Here, we present a protocol to generate tissue engineered vessel grafts that are functional for grafting into mice by double seeding partially induced pluripotent stem cell (PiPSC) - derived smooth muscle cells and PiPSC - derived endothelial cells on a decellularized vessel scaffold bioreactor.
10:32
Athymic Rat Model for Evaluation of Engineered Anterior Cruciate Ligament Grafts
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Cited by 11 •
2015
Animal models are important tools for the evaluation of tissue-engineered grafts. This paper presents the protocol for preparing an electrospun biodegradable polymer graft for use in anterior cruciate ligament tissue engineering, as well as a surgical protocol for implantation in a rat model.
FAQs
Covalent bond formation determines how firmly the added component remains attached and which functional groups become available at the interface. In practice, the chemistry of the participating groups influences the resulting surface or molecular environment, allowing attachment to modify compatibility, stability, or reactivity while preserving the original polymer backbone, surface, or scaffold.
Activation creates or exposes reactive sites on a surface, polymer backbone, or molecular scaffold. Those sites provide locations where the incoming component can form covalent bonds rather than remaining only associated with the original material. The activation step therefore helps make attachment chemically feasible and supports the introduction of selected functions into an existing framework.
Grafting changes an existing framework by adding a chemically attached component, whereas replacement would remove or substitute the original structure. This distinction matters because the parent surface, backbone, or scaffold can retain its underlying role while gaining new chemical behavior. Such modification enables property adjustment without redesigning the entire material system.
A general workflow begins by selecting the surface, polymer backbone, or molecular scaffold to be modified. The framework is then activated with a suitable reactive species, followed by contact with a component bearing compatible functional groups. Covalent attachment produces the modified architecture, which can then be examined for changes in surface chemistry, compatibility, stability, or reactivity.
The principal components are an existing framework, a reactive species used for activation, and an added component containing compatible functional groups. The framework may be a surface, polymer backbone, or molecular scaffold. Their chemical compatibility governs whether attachment can occur and helps determine which functions the completed system presents.
Applications include polymer functionalization, surface engineering, catalysis, and advanced-material development. In these settings, controlled attachment can improve compatibility or stability, alter surface chemistry, or introduce selective chemical functions. For catalytic and materials research, the modified architecture is useful when researchers need new reactivity or interfacial behavior without discarding the original framework.