Nasopharyngeal Swab

A nasopharyngeal swab is a clinical specimen-collection method that samples the upper respiratory tract to support laboratory diagnosis of respiratory infections. A flexible, synthetic-tipped swab is gently passed through the nostril to the nasopharynx, where it contacts mucosal secretions and collects cells, viral particles, or other microbial material before removal and placement in transport medium. Laboratories can analyze the specimen using molecular assays, antigen tests, or culture to identify pathogens such as respiratory viruses and guide infection control and patient management. Proper technique, specimen handling, and timely transport are essential for reliable results.

Nasopharyngeal Swab - Related Videos

Research

JoVE Journal - Immunology and Infection

Swab Sampling Method for the Detection of Human Norovirus on Surfaces

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Cited by 40 •

2017

A macrofoam based sampling methodology was developed and evaluated for the detection and quantification of norovirus on environmental hard surfaces.

Education

JoVE Core - Medical-Surgical Nursing

Suctioning the Nasopharyngeal Airway

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2025

Nasopharyngeal suctioning is a procedure to remove secretions from the upper part of the respiratory tract that the patient cannot clear independently. It helps maintain airway patency and prevents complications such as aspiration pneumonia. Equipment Required Suction machine with adjustable pressure settings Sterile suction catheters (appropriate size for the patient) Sterile gloves Sterile saline or water (for irrigation or lubrication) Water-soluble lubricant Personal protective...

Quantification of Group B Streptococcus Load in the Mouse Vaginal Lumen via Swabbing and Chromogenic Culture

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2026

Source: Patras, K. A. & Doran, K. S. A Murine Model of Group B Streptococcus Vaginal Colonization. J. Vis. Exp. (2016)This video demonstrates the quantification of vaginal Group B Streptococcus (GBS) load in a mouse model using swabbing and chromogenic culture. It outlines the steps involved in sample collection, plating, and colony counting to quantify GBS load in the mouse vaginal lumen.

Research

JoVE Journal - Immunology and Infection
Free Sample

Examining the Role of Nasopharyngeal-associated Lymphoreticular Tissue (NALT) in Mouse Responses to Vaccines

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Cited by 39 •

2012

Methods to examine contributions of the nasopharyngeal-associated lymphoreticular tissues (NALT) to nasal and systemic immune responses of mice to intranasal vaccines are described. We demonstrate a surgical procedure to establish a NALT-dependent mouse model and ex vivo cultures of extracted NALT.

Swab-based Conjunctival Commensal Bacteria Isolation: A Technique to Isolate Commensal Bacteria from Conjunctiva of Murine Model

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2025

This video describes a swab-based technique to isolate viable commensal bacteria from the ocular conjunctiva. The technique can be used to profile the commensal bacterial microbiome of the conjunctiva, which plays a defensive role against pathogens.

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