Spatula Brush
Spatula Brush - Related Videos
06:56
Particles without a Box: Brush-first Synthesis of Photodegradable PEG Star Polymers under Ambient Conditions
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Cited by 17 •
2013
Poly(ethylene glycol) (PEG) brush-arm star polymers (BASPs) with narrow mass distributions and tunable nanoscopic sizes are synthesized in via ring opening metathesis polymerization (ROMP) of a PEG-norbornene macromonomer followed by transfer of portions of the resulting living brush initiator to vials containing varied amounts of a rigid, photo-cleavable bis-norbornene crosslinker.
07:16
Spatula Montevideo Device for the Vitrification of Mammalian Embryos
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Cited by 1 •
2025
Here, we present the Spatula Montevideo protocol for vitrifying mouse and sheep pre-implantation embryos, consisting of a homemade spatula and utilizing homemade solutions. Embryos are rapidly equilibrated in two cryoprotectant solutions of increasing concentration, loaded onto the spatula, immersed in liquid nitrogen, and finally sealed with a 0.5 mL straw.
13:57
Preparation and Friction Force Microscopy Measurements of Immiscible, Opposing Polymer Brushes
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Cited by 4 •
2014
The methodology to perform friction force microscopy experiments for contacting brushes is presented: Two polymer brushes that are grafted from (a) substrates and (b) colloidal probes are slid to show that, by using two contacting immiscible brush systems, friction in sliding contacts is reduced compared to miscible brush systems.
04:30
A Flame-Free Method for Sterilizing C. elegans Picks, Spatulas, and Scalpels
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Cited by 1 •
2022
This article describes a method for sterilizing worm picks, spatulas, and scalpels using a micro-incinerator in place of an open flame.
10:25
Isolation and Quantitative Evaluation of Brush Cells from Mouse Tracheas
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Cited by 9 •
2019
Brush cells are rare cholinergic chemosensory epithelial cells found in the naïve mouse trachea. Due to their limited numbers, ex vivo evaluation of their functional role in airway immunity and remodeling is challenging. We describe a method for isolation of tracheal brush cells by flow cytometry.
FAQs
The spatula and brush provide complementary access to cervical surfaces rather than duplicating the same collection area. The spatula gathers exfoliated cells from the ectocervix, while the brush reaches the endocervical canal. Combining those cellular contributions can produce a sample that better represents the cervix for subsequent cytological assessment than relying on either component alone.
The sampled site matters because the ectocervical surface and endocervical canal are separate anatomical regions. In a Spatula Brush collection, the spatula addresses the external cervical surface, while the brush enters the canal and gently rotates to dislodge cells there. This division helps include material from both locations in one cytology sample.
Gentle rotation gives the brush a controlled way to contact the endocervical canal and dislodge additional exfoliated cells. Its purpose is therefore complementary to the spatula’s collection from the ectocervical surface, not simply to repeat it. Including these canal-derived cells can increase the anatomical coverage of the specimen submitted for cytological evaluation.
Collection proceeds by using the spatula at the ectocervical surface and the brush within the endocervical canal. The brush is gently rotated to dislodge cells, after which the collected material is transferred to either a glass slide or a liquid-based collection medium. These steps create the specimen used for laboratory cytological assessment.
Clinical screening is a central application because the collected cells can be examined for cellular abnormalities, including precancerous changes. The specimen supplies material for cytological evaluation rather than serving as the evaluation itself. This makes the device relevant to workflows in which cervical cells must be collected before laboratory assessment.
After collection, the material can be transferred to a glass slide or to a liquid-based collection medium. These are two specimen formats identified for subsequent cytological evaluation, so the sample can be prepared for laboratory assessment without changing the collection principle. This flexibility also supports use in both clinical screening and gynecologic research.