Isolation and Quantitative Evaluation of Brush Cells from Mouse Tracheas

Journal

Immunology and Infection

JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection

Isolation and Quantitative Evaluation of Brush Cells from Mouse Tracheas

DOI:

10.3791/59496-v

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10:25 min

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June 12, 2019

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Saltanat Ualiyeva, Eri Yoshimoto, Nora A. Barrett, Lora G. Bankova

¹Division of Rheumatology, Allergy and Immunology, Brigham and Women’s Hospital,Harvard Medical School

Chapters

00:04Title
01:24Preparation of Reagents
02:34Dissection of Mouse Trachea
04:47Tracheal Epithelial Digestion
08:31Results: Analysis of the Isolated Brush Cells from Mouse Tracheas
09:57Conclusion

Summary

Automatic Translation

Brush cells are rare cholinergic chemosensory epithelial cells found in the naïve mouse trachea. Due to their limited numbers, ex vivo evaluation of their functional role in airway immunity and remodeling is challenging. We describe a method for isolation of tracheal brush cells by flow cytometry.

Isolation and Quantitative Evaluation of Brush Cells from Mouse Tracheas

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