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JoVE Journal
Immunology and Infection

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Isolation and Quantitative Evaluation of Brush Cells from Mouse Tracheas
 

Isolation and Quantitative Evaluation of Brush Cells from Mouse Tracheas

Article DOI: 10.3791/59496
June 12th, 2019

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Summary June 12th, 2019

Brush cells are rare cholinergic chemosensory epithelial cells found in the naïve mouse trachea. Due to their limited numbers, ex vivo evaluation of their functional role in airway immunity and remodeling is challenging. We describe a method for isolation of tracheal brush cells by flow cytometry.

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