Topical Drug Permeation
Topical Drug Permeation - Related Videos
01:58
Studying the Permeation of FITC and FITC-Ferritin through an In Vitro Blood-Brain Barrier Model
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2025
This video demonstrates a method to study permeation through an in vitro blood-brain barrier (BBB) model using astrocytes and endothelial cells on a membrane insert, with free FITC and FITC-loaded ferritin. FITC-ferritin bound to receptors on endothelial cells and crossed the membrane barrier to the lower chamber while free FITC showed restricted permeation and remained in the upper chamber.
11:13
In Vitro Permeation of FITC-loaded Ferritins Across a Rat Blood-brain Barrier: a Model to Study the Delivery of Nanoformulated Molecules
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Cited by 15 •
2016
A method to establish an in vitro model of blood-brain barrier based on a co-culture of rat brain microvascular endothelial cells and astrocytes is described and validated. This system proved to be a valid tool to study the effect of nanoformulation on the trans-barrier permeation of fluorescent molecules.
04:43
Topical Testing Assay: A Technique to Evaluate the Insecticidal Potential of Dopamine Receptor Antagonists on Adult Female Mosquitoes by Topical Application
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2025
In this video, we demonstrate the topical testing assay to determine the toxicity of a potential insecticide on adult female mosquitoes. Using a dopamine receptor antagonist that causes lethal inhibition of neurotransmission in mosquitoes, this method describes a way to evaluate the toxic effects of insecticides upon topical application.
11:44
Preparing and Administering Topical Medications
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2017
Source: Madeline Lassche, MSNEd, RN and Katie Baraki, MSN, RN, College of Nursing, University of Utah, UT Topical medications are applied directly to the body surfaces, including the skin and mucous membranes of the eyes, ears, nose, vagina, and rectum. There are many classes of topical medications, such as creams, ointments, lotions, patches, and aerosol sprays. Medications that are applied to the skin to produce a slow, controlled, systemic effect are also referred to as transdermal.
08:44
Generation of Topically Transgenic Rats by In utero Electroporation and In vivo Bioluminescence Screening
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Cited by 7 •
2013
Genes can be manipulated during development of the cortex or the hippocampus of the rat via in utero electroporation (IUE) at E16, to enable fast and targeted modifications in neuronal connectivity for later studies of behavior or neuropathology in adult animals. Postnatal in vivo imaging for control of IUE success is performed by bioluminescence of activating co-transfected luciferase.
FAQs
Partitioning into the stratum corneum is a critical gateway for movement beyond the formulation. The active ingredient must first dissolve in the vehicle and then enter this outer skin layer before diffusing through deeper tissue. Its molecular properties and the formulation determine how readily that transfer occurs, affecting whether exposure remains near the application site or extends further.
Molecular properties, formulation design, skin condition, and application site all influence the amount and depth of drug movement. Changes in any of these factors can alter dissolution in the vehicle, entry into the stratum corneum, or subsequent diffusion through skin layers. Consequently, the same active ingredient may produce different permeation behavior in different products or clinical settings.
The application site matters because skin characteristics differ across locations, changing how an active ingredient moves through the tissue. These differences can affect the balance between local delivery, deeper-tissue exposure, and entry into systemic circulation. Considering site-specific behavior helps clinical researchers evaluate whether a formulation is likely to provide the intended therapeutic exposure while limiting unwanted effects.
These studies evaluate product performance by examining how an active pharmaceutical ingredient moves from a formulation through skin layers. They can reveal whether a product supports predominantly local treatment or permits deeper or systemic exposure. Such information helps researchers compare formulations, identify performance differences, and assess whether a candidate product warrants further development for its intended clinical use.
Permeation evaluations help researchers optimize different delivery formats by showing how formulation characteristics influence drug movement through skin. Results can guide adjustments intended to improve local treatment, support access to deeper tissues, or limit unnecessary systemic exposure. This approach is relevant across creams, gels, and patches because each format may produce a different delivery profile for the same active ingredient.
Findings can inform predictions about therapeutic exposure and help balance treatment effectiveness against unwanted effects. In dermatological research, they support selection and refinement of products intended to act at the skin or in deeper tissues. More broadly, the results contribute to decisions about product performance and the suitability of a formulation for further clinical development.