Ex Ovo Chick Embryo
Ex Ovo Chick Embryo - Related Videos
06:48
Chick ex ovo Culture
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2023
One strength of the chicken (Gallus gallus domesticus) as a model organism for developmental biology is that the embryo develops outside the female and is easily accessible for experimental manipulation. Many techniques allow scientists to examine chicken embryos inside the eggshell (in ovo), but embryonic access can be limited at later stages of development. Fortunately, chicks can also be cultured ex ovo, or outside of the eggshell. The major advantage to ex ovo culture is greater access to...
17:23
Chick ex ovo Culture and ex ovo CAM Assay: How it Really Works
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Cited by 110 •
2009
The chick chorioallantoic membrane (CAM) is a unique, naturally immunodeficient supportive culture environment to study angiogenesis and tumorigenesis. This video article demonstrates the different steps in chick ex ovo culture, application of potentially angiogenic substances and successful inoculation of tumor cells and tissues on the surface of the CAM.
06:57
In ovo Electroporation of Chicken Embryos
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2023
Electroporation is a technique used in biomedical research that allows for the manipulation of gene expression via the delivery of foreign genetic material into cells. More specifically, in ovo electroporation is performed on early developing chicks (Gallus gallus domesticus) contained within their eggshells. In this procedure, DNA or knockdown constructs are first injected into a target tissue. However, the genetic material is unable to penetrate the plasma membrane to carry out its function...
08:57
In ovo Electroporation in Chick Midbrain for Studying Gene Function in Dopaminergic Neuron Development
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Cited by 5 •
2012
To assess the function and the regulation of genes during the development of midbrain dopaminergic neurons, we describe a method that involves in ovo electroporation of plasmid DNA constructs into embryonic chick ventral midbrain dopaminergic neuron progenitors. This technique can be used to achieve efficient expression of genes of interest to study different aspects of midbrain development and dopaminergic neuron differentiation.
13:06
In Ovo Electroporations of HH Stage 10 Chicken Embryos
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Cited by 19 •
2007
Chick in ovo electroporation is a technique which allows genetic manipulation of the avian embryo. Common applications of this technique include functional analysis of genes and putative enhancer elements. This video demonstrates neural tube electroporation in HH 10 chick embryos. Injection technique and proper egg handling are discussed.
FAQs
The yolk and albumen provide the embryo with its immediate supporting environment after removal from the shell. In an ex ovo culture, these materials remain with the embryo inside a transparent vessel, while temperature, humidity, and gas exchange are controlled. Preserving this arrangement helps sustain development while keeping the living tissue accessible for study.
Removing the shell eliminates a physical barrier that would otherwise limit direct viewing and manipulation. Researchers can observe developmental changes more readily and perform microsurgical interventions on exposed embryonic tissues. This access supports experiments that examine how specific physical, chemical, or genetic perturbations influence tissue formation and organ development during embryogenesis.
Temperature, humidity, and gas exchange are central conditions because they sustain development outside the eggshell. The culture vessel must therefore provide a controlled environment rather than simply expose the embryo to air. Changes in these conditions can affect whether development continues appropriately, making environmental control essential when comparing experimental and unperturbed embryos.
The embryo is transferred from its eggshell together with the yolk and supporting albumen into a transparent culture vessel. The vessel is then maintained under controlled temperature, humidity, and gas-exchange conditions. Once established, the exposed embryo can be observed directly and made available for imaging, microsurgery, or other developmental experiments.
This model supports real-time imaging and microsurgical intervention, allowing researchers to follow living embryonic tissues as they develop. It also provides a setting for testing genetic, chemical, or physical perturbations. Resulting changes can be assessed through effects on tissue formation, organ development, vascular growth, and overall embryonic outcomes.
The system combines direct access to a living vertebrate embryo with relatively low cost and straightforward observation. In developmental biology, that combination makes it useful for investigating mechanisms of embryogenesis, including the formation of tissues, organs, and blood vessels. Researchers can connect visible developmental changes with experimental perturbations in a controlled culture environment.