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Neuroscience

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In ovo Electroporation in Chick Midbrain for Studying Gene Function in Dopaminergic Neuron Development
 

In ovo Electroporation in Chick Midbrain for Studying Gene Function in Dopaminergic Neuron Development

Article doi: 10.3791/4017
August 3rd, 2012

Summary August 3rd, 2012

To assess the function and the regulation of genes during the development of midbrain dopaminergic neurons, we describe a method that involves in ovo electroporation of plasmid DNA constructs into embryonic chick ventral midbrain dopaminergic neuron progenitors. This technique can be used to achieve efficient expression of genes of interest to study different aspects of midbrain development and dopaminergic neuron differentiation.

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