Ex Vivo Tissue
Ex Vivo Tissue - Related Videos
09:55
Tissue Engineering by Intrinsic Vascularization in an In Vivo Tissue Engineering Chamber
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Cited by 17 •
2016
This is a guideline for constructing in vivo vascularized tissue using a microsurgical arteriovenous loop or a flow-through pedicle configuration inside a tissue engineering chamber. The vascularized tissues generated can be employed for organ regeneration and replacement of tissue defects, as well as for drug testing and disease modeling.
12:08
Ex Vivo Infection of Live Tissue with Oncolytic Viruses
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Cited by 22 •
2011
Oncolytic viruses are promising for cancer therapeutics. The ability to ascertain the infectability of live tissue specimens obtained from patients prior to treatment is a unique advantage of this therapeutic approach. This protocol describes how to process tissues for ex vivo infection with oncolytic virus and subsequent viral quantification.
05:00
Ex Vivo Culture of Patient Tissue & Examination of Gene Delivery
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Cited by 4 •
2010
This article describes the culture of patient tissue slices for gene delivery studies and subsequent analysis of gene expression using IVIS bioluminescence imaging.
11:17
Surgical Technique for the Implantation of Tissue Engineered Vascular Grafts and Subsequent In Vivo Monitoring
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Cited by 9 •
2015
A step-by-step protocol for the inter-positional placement of Tissue Engineered Vessels (TEVs) into the carotid artery of a sheep using end-to-end anastomosis and real-time digital assessment in vivo until animal sacrifice.
10:00
2.5D Model for Ex Vivo Mechanical Characterization of Sprouting Angiogenesis in Living Tissue
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2025
Sprouting angiogenesis, fundamental for development and disease, involves complex molecular and mechanical processes. We present a versatile 2.5D ex vivo model that analyzes cellular sprouting from porcine carotid arteries, revealing stiffness-dependent angiogenesis and distinct leader-follower cell mechanics. This model aids in advancing tissue engineering strategies and cancer therapy approaches.
FAQs
Nutrients, oxygen, temperature, and signaling conditions jointly determine whether the tissue stays viable and retains meaningful biological behavior. These factors must support both cell survival and the tissue’s organization or function. In developmental studies, controlling them helps distinguish genuine growth, differentiation, or morphogenetic responses from changes caused by inadequate laboratory maintenance.
Signaling conditions help preserve or alter the cues that regulate growth, differentiation, morphogenesis, and interactions among neighboring cells. Adjusting these conditions gives researchers a way to examine how tissue responses change under controlled circumstances. This makes it possible to connect observed developmental outcomes with specific signaling environments while limiting the complexity of the whole organism.
Native cellular organization maintains spatial relationships that can influence cell-to-cell interactions, tissue growth, and morphogenesis. When this organization remains intact, researchers can study developmental behavior in a setting that retains important tissue-level context. The resulting observations may reveal relationships that would be difficult to interpret from isolated cells or from measurements that lack direct spatial information.
A typical workflow begins by removing tissue from the organism, placing it under controlled laboratory conditions, and supplying the nutrients, oxygen, temperature, and signaling support needed for viability. Researchers then observe or manipulate the preparation while monitoring developmental responses such as growth, differentiation, morphogenesis, cellular interactions, or changes associated with gene function.
These preparations can provide direct observations of growth, differentiation, morphogenesis, and cell-to-cell interactions. Because the tissue is accessible outside the organism, researchers can combine controlled manipulation with precise imaging to follow developmental responses. The resulting information describes both tissue behavior and responses to experimental conditions, while retaining more organization than many simpler cellular systems.
Ex vivo tissue is especially useful when researchers need precise imaging, direct manipulation, or tighter control over the experimental environment. It reduces the complexity of the whole organism while preserving selected tissue-level features. For developmental biology, the approach can test mechanisms and gene function before findings are compared with, or complemented by, in vivo experiments.