Zebrafish Blood Development
Zebrafish Blood Development - Related Videos
08:07
Zebrafish Reproduction and Development
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2023
The zebrafish (Danio rerio) has become a popular model for studying genetics and developmental biology. The transparency of these animals at early developmental stages permits the direct visualization of tissue morphogenesis at the cellular level. Furthermore, zebrafish are amenable to genetic manipulation, allowing researchers to determine the effect of gene expression on the development of a vertebrate with a high degree of genetic similarity to humans. This video provides a brief overview of...
06:17
Repeated Blood Collection for Blood Tests in Adult Zebrafish
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Cited by 87 •
2015
Repeated blood sampling is necessary and important in animal research. We developed a novel, non-lethal and reliable method for repeated blood collection from adult zebrafish, and applied this method to the study of blood biochemistry, including glucose metabolism.
03:11
Blood Collection for Biochemical Analysis in Adult Zebrafish
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Cited by 111 •
2012
This paper presents a technique for collection of blood from the dorsal aorta of Zebrafish. It also provides instructions for obtaining serum for use in biochemical analyses, such as tests for determining cholesterol and triglyceride levels.
07:32
Using Flow Cytometry to Detect and Quantitate Altered Blood Formation in the Developing Zebrafish
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Cited by 4 •
2021
This assay is a simple method to quantitate hematopoietic cells in developing embryonic zebrafish. Blood cells from dissociated zebrafish are subjected to flow cytometry analysis. This allows the detection of blood defects in mutant animals and after genetic modification.
10:38
Generation of Parabiotic Zebrafish Embryos by Surgical Fusion of Developing Blastulae
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Cited by 12 •
2016
This protocol provides step-by-step instruction on how to generate parabiotic zebrafish embryos of different genetic backgrounds. When combined with the unparalleled imaging capabilities of the zebrafish embryo, this method provides a uniquely powerful means to investigate cell-autonomous versus non-cell-autonomous functions for candidate genes of interest.
FAQs
Primitive and definitive hematopoiesis represent distinct developmental phases. Mesodermal cells first produce primitive erythrocytes and myeloid cells. Later, definitive hematopoietic stem and progenitor cells emerge in the aorta-gonad-mesonephros region. This distinction lets developmental biologists compare early blood-cell production with the stem-cell-generating process that supports later hematopoietic development.
The aorta-gonad-mesonephros region is important because it is where definitive hematopoietic stem and progenitor cells arise. After emerging, these cells enter the circulation and seed the kidney marrow, which functions as the adult hematopoietic organ in zebrafish. Following this sequence helps researchers connect embryonic stem-cell production with the establishment of lifelong blood-forming tissue.
Vascular development provides a developmental context for blood formation and movement. The embryonic blood-forming process is therefore studied alongside the vessels through which definitive stem and progenitor cells enter circulation. Examining these linked events helps clarify how cell fate specification, tissue formation, and migration are coordinated during development.
Researchers examine transparent zebrafish embryos across embryonic stages, using fluorescent reporters and live imaging to follow blood development in real time. This approach provides a direct view of when blood cells appear and how developing tissues contribute to hematopoiesis during embryogenesis, making successive developmental events accessible without relying only on endpoint observations.
The model supports investigation of conserved mechanisms of hematopoiesis, including relationships between blood-cell formation, tissue development, and migration. It also enables researchers to model blood disorders and evaluate candidate therapies in a vertebrate embryo. These uses connect developmental observations with disease-related and treatment-oriented questions.
In developmental biology, zebrafish blood development provides a way to study how cell fate, vascular formation, and hematopoietic organization unfold together. Embryonic transparency and real-time imaging make successive stages accessible, while definitive stem and progenitor cells can be related to their later seeding of the kidney marrow, the adult blood-forming organ.