Gmr-gal4 Uas-alpha-catenin-gfp
Gmr-gal4 Uas-alpha-catenin-gfp - Related Videos
06:29
Using the GAL4-UAS System for Functional Genetics in Anopheles gambiae
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Cited by 11 •
2021
The bipartite GAL4-UAS system is a versatile tool for modification of gene expression in a controlled spatiotemporal manner which permits functional genetic analysis in Anopheles gambiae. The procedures described for using this system are a semi-standardized cloning strategy, sexing and screening of pupae for fluorescent protein markers and embryo fixation.
13:34
Gene Trapping Using Gal4 in Zebrafish
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Cited by 19 •
2013
This protocol describes the method of gene trap insertional mutagenesis using Gal4-VP16 as the primary reporter and GFP/RFP as secondary reporters in zebrafish. Approximately one in ten high-expressing F0 fish yield gene trap progeny co-expressing GFP and RFP. The screening procedure can be readily scaled to adapt to the size of the laboratory performing the insertional mutagenesis screen.
08:52
Live Imaging of Innate Immune and Preneoplastic Cell Interactions Using an Inducible Gal4/UAS Expression System in Larval Zebrafish Skin
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Cited by 9 •
2015
Studying the earliest events of preneoplastic cell progression and innate immune cell interaction is pivotal to understand and treat cancer. Here we describe a method to conditionally induce epithelial cell transformations and the subsequent live imaging of innate immune cell interaction with HRASG12V expressing skin cells in zebrafish larvae.
01:23
Catenins
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2023
Catenins are characterized by multiple binding domains and dynamic structures that allow them to function as linker proteins in cell junction complexes. All catenins, except α-catenin, contain a characteristic protein sequence called the armadillo repeat and are therefore also called armadillo proteins. Catenins in Cell Junctions Catenins bind to cell adhesion molecules such as cadherins and link them to different cytoskeletal proteins depending on the type of cell junction. At the adherens...
09:41
Reconstitution Of β-catenin Degradation In Xenopus Egg Extract
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Cited by 4 •
2014
A method is described for analyzing protein degradation using radiolabeled and luciferase-fusion proteins in Xenopus egg extract and its adaptation for high-throughput screening for small molecule modulators of protein degradation.
FAQs
The GMR promoter restricts GAL4 activity to developing retinal tissues, while the UAS sequence responds to that GAL4 driver. This separates tissue targeting from production of the alpha-catenin-GFP fusion. Consequently, fluorescence reports adhesion-related structures in the eye rather than providing a general, organism-wide signal, allowing developmental changes to be examined in a defined tissue context.
Alpha-catenin is positioned at the interface between cadherin-based adherens junctions and the actin cytoskeleton. Fusing it to GFP therefore links a structural adhesion component with a visible fluorescent readout. Changes in the distribution or appearance of the signal can help investigators examine junctional organization, cell shape, and adhesion-related remodeling during retinal development.
The fluorescent fusion can expose how altered adhesion influences epithelial organization and tissue morphogenesis. In the developing eye, those effects can be considered alongside changes in ommatidial patterning, the arrangement of the repeating visual units. This makes the system useful for connecting molecular or cellular adhesion changes with larger-scale developmental organization.
Researchers can analyze the alpha-catenin-GFP signal in either living eye tissues or fixed preparations, depending on the developmental question. Live analysis supports examination of junctional dynamics and changing cell shapes, whereas fixed tissue can provide a preserved view of epithelial organization and ommatidial patterning at a selected developmental state.
It is especially useful when a study focuses on adhesion within developing retinal epithelium and needs a readout tied to tissue structure. Investigators can use the system to examine how gene-function changes affect cell adhesion, epithelial organization, cell shape, or ommatidial patterning, thereby connecting genetic perturbations with visible morphogenetic outcomes in the eye.
Interpretation should consider several related features rather than fluorescence alone. Researchers can compare epithelial organization, cell shape, junctional dynamics, tissue morphogenesis, and ommatidial patterning across experimental conditions. Together, these observations help determine whether a genetic change is associated with altered adhesion and whether the effect remains local to junctions or extends to overall eye-tissue organization.