Muscle Injury
Muscle Injury - Related Videos
07:13
Laser-inflicted Injury of Zebrafish Embryonic Skeletal Muscle
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Cited by 14 •
2013
The method presented here comprises the precise injury of live zebrafish embryos with high-energy laser pulses and the subsequent analysis of these injuries and their recovery with time. We also show how genetically labeled single or groups of skeletal muscle cells can be tracked during and after laser light induced damage.
06:46
In Vivo Functional Assessment of Rat Masseter Muscle Following Surgical Creation of a Volumetric Muscle Loss (VML) Injury
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Cited by 1 •
2024
Volumetric muscle loss (VML) injuries exceed endogenous regenerative ability, resulting in permanent functional deficits. Current VML research primarily focuses on limb and trunk muscles. To extend mechanistic studies of VML to craniofacial muscles, this article describes an in vivo method for longitudinal assessment of masseter muscle function pre- and post-VML injury.
09:37
Evaluation of Respiratory Muscle Activation Using Respiratory Motor Control Assessment (RMCA) in Individuals with Chronic Spinal Cord Injury
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Cited by 18 •
2013
The purpose of this publication is to present our original work on a multi-muscle surface electromyographic approach to quantitatively characterize respiratory muscle activation patterns in individuals with chronic spinal cord injury using vector-based analysis.
09:14
Evaluation of Injury-induced Senescence and In Vivo Reprogramming in the Skeletal Muscle
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Cited by 14 •
2017
Here we present a detailed protocol to detect both senescent and pluripotent stem cells in the skeletal muscle upon injury while inducing in vivo reprogramming. This method is suitable for evaluating the role of cellular senescence during tissue regeneration and reprogramming in vivo.
06:14
Murine Aortic Crush Injury: An Efficient In Vivo Model of Smooth Muscle Cell Proliferation and Endothelial Function
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Cited by 2 •
2017
Restenosis following cardiovascular procedures (bypass surgery, angioplasty, or stenting) is a significant problem reducing the durability of these procedures. An ideal therapy would inhibit smooth muscle cell (VSMC) proliferation while promoting regeneration of the endothelium. We describe a model for simultaneous assessment of VSMC proliferation and endothelial function in vivo.
FAQs
Damaged skeletal muscle fibers and surrounding tissue release signals that recruit inflammatory cells to the injured area. This response connects local damage with activation of resident satellite cells. The satellite cells then proliferate and differentiate, creating a progression from injury recognition to tissue rebuilding that can ultimately support restored force production and function.
Satellite cells provide a model for studying how muscle progenitors are controlled during tissue formation, growth, and regeneration. Their activation, proliferation, and differentiation reveal how developmental programs can be re-engaged after damage. This connection helps researchers examine how progenitor behavior contributes to effective repair, developmental defects, or incomplete recovery.
The extracellular matrix provides an essential context for coordinating muscle repair with cellular behavior. In developmental biology, researchers examine how matrix interactions work together with muscle progenitors and signaling pathways during tissue formation and regeneration. This perspective helps distinguish organized rebuilding that supports function from repair associated with fibrosis and impaired recovery.
Researchers compare whether the response rebuilds functional muscle tissue or produces fibrosis, an outcome associated with excessive or incomplete tissue repair. Restoration of force production and function indicates more effective recovery, whereas persistent structural disruption or fibrotic replacement suggests that repair has not fully recreated the original muscle organization.
A complete analysis considers damaged fibers, signals that recruit inflammatory cells, satellite-cell activation and expansion, progenitor differentiation, extracellular-matrix behavior, and changes in force production. Examining these features together reveals how the response progresses from injury through regeneration and helps identify where tissue rebuilding becomes incomplete or shifts toward fibrosis.
These models can be used to investigate how muscle progenitors, extracellular matrix, and signaling pathways coordinate tissue formation, growth, and regeneration. They also help researchers study congenital muscle disorders and developmental defects by comparing normal repair processes with responses that fail to restore muscle structure or function.
Muscle injury research identifies cellular and tissue processes that support regeneration, including inflammatory recruitment, satellite-cell behavior, progenitor differentiation, and matrix coordination. Understanding these interactions can guide regenerative medicine research aimed at restoring muscle function. It also highlights the need to promote effective rebuilding while limiting fibrosis and incomplete recovery.