Platinum Shadowing
Platinum Shadowing - Related Videos
08:41
Single Wavelength Shadow Imaging of Caenorhabditis elegans Locomotion Including Force Estimates
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Cited by 2 •
2014
The technique presented here measures the path of freely swimming microscopic species using single wavelength exposure. C. elegans are used to demonstrate shadow imaging as an inexpensive alternative to costly microscopes. This technique can be adapted to accommodate various orientations, environments and species to measure direction, speed, acceleration and forces.
07:20
Amide Coupling Reaction for the Synthesis of Bispyridine-based Ligands and Their Complexation to Platinum as Dinuclear Anticancer Agents
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Cited by 1 •
2014
This protocol describes the use of amide coupling reactions of isonicotinic acid and diaminoalkanes to form bridging ligands suitable for use in the synthesis of multinuclear platinum complexes, which combine aspects of the anticancer drugs BBR3464 and picoplatin.
08:32
Electrochemical Roughening of Thin-Film Platinum Macro and Microelectrodes
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2019
This protocol demonstrates a method for electrochemical roughening of thin-film platinum electrodes without preferential dissolution at grain boundaries. The electrochemical techniques of cyclic voltammetry and impedance spectroscopy are demonstrated to characterize these electrode surfaces.
09:02
Synthesis of Platinum-nickel Nanowires and Optimization for Oxygen Reduction Performance
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2018
The protocol describes the synthesis and electrochemical testing of platinum-nickel nanowires. Nanowires were synthesized by the galvanic displacement of a nickel nanowire template. Post-synthesis processing, including hydrogen annealing, acid leaching, and oxygen annealing were used to optimize nanowire performance and durability in the oxygen reduction reaction.
09:40
Tropomodulin 3 Overexpression as a Marker for Platinum Resistance and Immune Infiltration in Ovarian Cancer
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Cited by 2 •
2024
Tropomodulin 3 (TMOD3) has been increasingly studied in tumors in recent years. This study is the first to report that TMOD3 is highly expressed in ovarian cancer and is closely associated with platinum resistance and immune infiltration. These results could help improve the therapeutic outcomes for ovarian cancer.
FAQs
The source is positioned at an oblique angle rather than directly above the specimen. Exposed surfaces intercept more evaporated platinum, whereas recessed regions receive less coating. This directional deposition creates differences in metal coverage that translate into visible surface relief during electron microscopy, allowing investigators to distinguish architectural features that would be harder to resolve from coating alone.
The distribution of platinum provides a visual indication of surface geometry rather than a uniform record of composition. Areas with heavier accumulation correspond to exposed regions, while weaker coverage marks recesses. Interpreting this pattern helps researchers reconstruct the three-dimensional organization of cellular, extracellular, or macromolecular structures from the way their surfaces interact with the directional coating.
A supporting carbon layer can stabilize the replica for imaging after platinum deposition. Its role is structural rather than contrast-producing: platinum supplies the differential surface coverage, while carbon helps preserve that coated representation as it is examined. This distinction helps researchers understand that carbon supports the coated replica, not the directional shadowing pattern used to reveal surface relief.
Preparation places the specimen in a high-vacuum chamber, positions the platinum source at an oblique angle, and evaporates platinum so that exposed and recessed regions receive different amounts of metal. A supporting carbon layer may then stabilize the replica for imaging. The resulting preparation is examined by electron microscopy to assess surface architecture and three-dimensional form.
The technique is useful when developmental biologists need to examine the shape and organization of cells, extracellular structures, or macromolecular assemblies at ultrastructural scale. By enhancing surface contrast, it supports analysis of architectural changes associated with tissue formation and morphogenesis. Its value is greatest when surface organization provides clues about how developing structures acquire their form.
Platinum shadowing can reveal surface features whose organization changes during development, allowing researchers to relate ultrastructural observations to tissue formation and morphogenesis. The images can show how cellular surfaces, extracellular structures, and macromolecular assemblies are arranged in three dimensions. These observations provide structural context for interpreting developmental changes rather than describing development only at the tissue level.