Umbilical Vein Cannulation
Umbilical Vein Cannulation - Related Videos
09:19
Isolation of Human Umbilical Vein Endothelial Cells (HUVEC)
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Cited by 131 •
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This video protocol illustrates the isolation and culture of human umbilical vein endothelial cells (HUVEC) from human umbilical cord. Once isolated these cells can be used for in vitro angiogenesis assays like the Optimized Fibrin Gel Bead Assay also demonstrated by the Hughes lab.
08:54
Isolation of Human Umbilical Vein Endothelial Cells and Their Use in the Study of Neutrophil Transmigration Under Flow Conditions
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This article first describes a procedure for isolating human endothelial cells from umbilical veins and then shows how to use these cells to examine neutrophil transmigration under flow conditions. By using a low-volume flow chamber made from a polymer with the optical characteristics of glass, live-cell fluorescent imaging of rare cell populations is also possible.
10:25
High-resolution Time-lapse Imaging and Automated Analysis of Microtubule Dynamics in Living Human Umbilical Vein Endothelial Cells
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07:51
Peripheral Venous Cannulation
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2023
Source: Sharon Bord, MD, Department of Emergency Medicine, The Johns Hopkins University School of Medicine, Maryland, USA Placement of an intravenous (IV) catheter is one of the key procedures in medicine. The IV catheter allows patients to receive critical medications, including pain medicine, insulin, antibiotics, blood products, and fluids for rehydration. Additionally, placing an IV catheter allows for blood samples to be obtained, which can be sent to the laboratory for testing and...
02:05
Intraduodenal Cannulation in a Rat Model for Immunopathology Studies
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2025
This video demonstrates intraduodenal cannulation in a rat model. After anesthetizing the rat, the duodenum is located and a small puncture is created. A J-shaped cannula with a beveled tip is carefully inserted and secured, and the incision is closed. The cannulated rat model enables intra-intestinal infusion for immunopathology studies.
FAQs
The catheter can be directed from the umbilical stump through the umbilical vein toward the ductus venosus and inferior vena cava. This route connects the accessible umbilical vessel with deeper fetal and neonatal circulation, making the technique relevant to studies of how blood flow is reorganized as the newborn transitions from fetal to postnatal life.
It provides an alternative route for vascular access immediately after birth, when clinicians may need to administer fluids, medications, or blood products without relying on peripheral vessels. The same access can support sample collection, giving clinicians and investigators a way to obtain material or deliver treatment during an early and physiologically important period.
The umbilical vein is part of a fetal circulation pathway that includes the ductus venosus and connects toward the inferior vena cava. Following this route with a catheter offers a practical way to examine circulation during the transition to neonatal life, including the early postnatal changes that distinguish fetal physiology from newborn physiology.
In developmental biology, the technique provides direct access to a vascular pathway associated with fetal circulation during the immediate postnatal period. That access helps investigators examine early postnatal physiology and clinically important adaptations after birth, linking developmental processes before birth with the functional changes that occur as neonatal life begins.
The basic workflow begins at the umbilical stump, where the catheter enters the umbilical vein. It may then be advanced toward the ductus venosus and inferior vena cava, depending on the intended access. Once positioned along this route, the catheter can be used to deliver fluids, medications, or blood products, or to collect samples.
This access supports several immediate postnatal needs: delivery of fluids, medications, and blood products, together with collection of vascular samples. Consequently, the technique serves both therapeutic and investigative purposes. It can provide a practical means of intervention when peripheral access is difficult while also enabling examination of early neonatal physiology.
Immediately after birth, circulation is undergoing a major physiological transition from the fetal state to neonatal life. Access through the umbilical vein is therefore especially informative during this window. It allows clinicians to support urgent vascular needs while giving developmental biology studies a way to investigate early postnatal function and adaptation.