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Immunology and Infection
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Isolation of Human Umbilical Vein Endothelial Cells and Their Use in the Study of Neutrophil Transmigration Under Flow Conditions
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Immunology and Infection
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JoVE Journal Immunology and Infection
Isolation of Human Umbilical Vein Endothelial Cells and Their Use in the Study of Neutrophil Transmigration Under Flow Conditions

Isolation of Human Umbilical Vein Endothelial Cells and Their Use in the Study of Neutrophil Transmigration Under Flow Conditions

DOI:
10.3791/4032-v

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08:54 min

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August 08, 2012

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, , , ,
1Department of Physiology and Pharmacology,University of Calgary

Chapters

  • 00:05Title
  • 01:41Isolation and Maintenance of Human Vascular Endothelial Cells
  • 04:54Neutrophil Recruitment and Transmigration
  • 06:36Results: Fluorescent and Cell Migration Analysis of Neutrophil Transmigration Across Endothelial Cells in an Ibidi Chamber
  • 08:03Conclusion

Summary

Automatic Translation

Automatic Translation

This article first describes a procedure for isolating human endothelial cells from umbilical veins and then shows how to use these cells to examine neutrophil transmigration under flow conditions. By using a low-volume flow chamber made from a polymer with the optical characteristics of glass, live-cell fluorescent imaging of rare cell populations is also possible.

Tags

IsolationHuman Umbilical Vein Endothelial CellsNeutrophil TransmigrationFlow ConditionsWhite Blood CellInnate Immune SystemAcute InflammationInjury SiteBlood VesselsMicrovascular Structural ChangesPlasma ProteinsRollingAdhesionAccumulationIn Vivo MethodsIn Vitro MethodsHuman NeutrophilsPrimary Human Umbilical Vein Endothelial Cells (HUVEC)Neutrophil IsolationConfluenceActivation MoleculesCytokines

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