Aggregate Degradation Measurement
Aggregate Degradation Measurement - Related Videos
12:49
Measurement of Aggregate Cohesion by Tissue Surface Tensiometry
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Cited by 10 •
2011
We describe a method of measuring binding energy, expressible as tissue surface tension, between cells within 3D tissue-like aggregates. Differences in tissue surface tension have been demonstrated to correlate with invasiveness of lung, muscle, and brain tumors, and are fundamental determinants of establishing spatial relationships between different cell types.
10:56
Assays for the Degradation of Misfolded Proteins in Cells
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Cited by 5 •
2016
This report describes protocols for measuring degradation rates of misfolded proteins by either western blot or fluorescence-based assays. The methods can be applied to analysis of other misfolded proteins and for high throughput screening.
05:42
Misfolding-Prone Protein Degradation Assay: A Technique to Monitor Misfolded Protein Degradation Using Cycloheximide Treatment and Detergent Fractionation
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2025
This video describes a degradation assay for misfolded proteins using cycloheximide treatment along with detergent fractionation. This method aids in studying the dynamics of misfolded proteins and uncovering the in-depth mechanisms of protein turnover.
02:58
Regulated Protein Degradation
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2020
It is vital to regulate the activity of enzymatic as well as non-enzymatic proteins inside the cell. This can be achieved either through creating a balance between their rate of synthesis and degradation or regulating the intrinsic activity of the protein. Both these regulation mechanisms play an essential role in the normal functioning of cells. Protein degradation plays two important roles in the cells. It helps to protect cells from misfolded or damaged proteins before they lead to a...
02:11
Proteins: From Genes to Degradation
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2020
Within a biological system, the DNA encodes the RNA, and the nucleotide sequence in the RNA further defines the amino acid sequence in the protein. This is referred to as “The Central Dogma of Molecular Biology” - a term coined by Francis Crick. Central dogma is a firm principle in biology that defines the flow of genetic information within any life form. The two fundamental steps in central dogma are - transcription and translation. Transcription is the synthesis of RNA molecules by RNA...
FAQs
Several measurements provide complementary evidence. Mass change indicates material loss, while particle-size distribution shows whether larger particles have broken into smaller fractions. Fines content specifically tracks the generation of small particles, and surface integrity reflects visible or measurable deterioration of particle surfaces. Using these indicators together gives engineers a more complete basis for comparing aggregate durability.
Mechanical action can damage aggregates through abrasion, impact, and crushing, whereas environmental conditioning exposes them to conditions associated with moisture or weathering. These mechanisms may alter different aspects of particle performance, including size distribution, fines generation, mass, or surface integrity. Separating the sources of deterioration helps engineers relate laboratory results to the loading and exposure conditions expected in service.
Particle-size distribution shows how degradation changes the proportion of coarse and finer material. A shift toward smaller particles can indicate that aggregate has fractured or worn during testing, even when total mass change alone provides limited detail. This information is especially relevant when aggregate performance depends on maintaining a specified gradation in concrete, asphalt, rail ballast, or other infrastructure.
No single indicator fully describes aggregate performance. Mass loss quantifies material removed from the sample, fines content identifies the accumulation of small degraded particles, and surface integrity indicates damage affecting the particle exterior. Comparing these outcomes helps distinguish limited surface wear from more substantial breakdown and supports more informed judgments about suitability, durability, and anticipated service behavior.
A typical workflow places aggregate particles under a defined combination of mechanical action, environmental conditioning, or repeated use, then evaluates the resulting changes. Engineers compare the material before and after testing by measuring mass, particle-size distribution, fines content, or surface integrity. The resulting data can be used to compare candidate aggregates and assess whether their degradation is acceptable for a project.
Engineers apply these measurements when selecting aggregates for concrete, asphalt, rail ballast, and other infrastructure exposed to traffic, loading, moisture, or weathering. The results support quality control by identifying differences in durability among materials. They also inform design and maintenance decisions, helping reduce premature failure when aggregate deterioration could impair performance during repeated service.