Anterior Segment Imaging

Anterior segment imaging is the noninvasive visualization and measurement of the eye’s front structures, including the cornea, iris, anterior chamber, and lens, supporting diagnosis, treatment planning, and device development. Depending on the modality, it uses reflected or transmitted light, optical sectioning, or interferometric measurements to produce two- or three-dimensional images and quantify tissue shape, thickness, and transparency. In engineering, these systems combine optical design, sensors, image processing, and computational analysis to improve resolution, speed, and reliability. Applications include evaluating corneal disease, planning refractive and cataract procedures, assessing intraocular lenses, and guiding the development of ophthalmic instruments.

Anterior Segment Imaging - Related Videos

Research

JoVE EoE - Large Animal Models

Anterior Eye Segment Dissection: A Method to Isolate Cornea Bearing Anterior Segment from Porcine Eye

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2025

In this video, we demonstrate the dissection of the cornea-bearing anterior segment of the porcine eye. The isolated segment can be used for organ culturing to study the pathophysiology of eye disorders.

Anterior Eye Segment Culturing: A Method to Culture Cornea Bearing Anterior Segment of Porcine Eye

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2025

In this video, we demonstrate the culturing method of a cornea-bearing anterior eye segment from a porcine eye. This culture system serves as an experimental model to study eye injuries.

Anterior Segment Organ Culture Platform for Tracking Open Globe Injuries and Therapeutic Performance

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Cited by 1 •

2021

Open globe eye injuries may go untreated for multiple days in rural or military-relevant scenarios, resulting in blindness. Therapeutics are needed to minimize loss of vision. Here, we detail an organ culture open globe injury model. With this model, potential therapeutics for stabilizing these injuries can be properly evaluated.

Research

JoVE Journal - Biology
Free Sample

Generation of Dispersed Presomitic Mesoderm Cell Cultures for Imaging of the Zebrafish Segmentation Clock in Single Cells

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Cited by 8 •

2014

Somitogenesis is a rhythmic developmental process that spatially patterns the body axis of vertebrate embryos. Previously, we developed transgenic zebrafish lines that use fluorescent reporters to observe the cyclic genes that drive this process. Here, we culture dispersed cells from these lines and image their oscillations over time in vitro.

Research

JoVE Journal - Medicine
Free Sample

Laparoscopic Anatomical Right Hemihepatectomy via the In Situ Anterior Approach

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2025

To evaluate the clinical benefits of laparoscopic right hemihepatectomy via the in situ anterior approach, emphasizing its advantages in minimizing tumor dissemination risks, reducing bleeding, and enhancing surgical safety, particularly for complex liver tumors.

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