Digital Microscope
Digital Microscope - Related Videos
10:28
Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization
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Cited by 5 •
2016
We present a compact reflection digital holographic system (CDHM) for inspection and characterization of MEMS devices. A lens-less design using a diverging input wave providing natural geometrical magnification is demonstrated. Both static and dynamic studies are presented.
06:06
Studying Cell Death Initiation Using a Digital Microscope
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2023
Here we present a protocol for studying the rate of programmed cell death initiation by continuously imaging inoculated leaves following cell death induction.
07:42
Imaging of Estrogen Receptor-α in Rat Pial Arterioles using a Digital Immunofluorescent Microscope
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2011
The goal of this article is to demonstrate a method to optimize immunofluorescent detection of estrogen receptor-α (ERα) in rat pial arterial slices using a digital immunofluorescent microscope.
08:08
Major Components of the Light Microscope
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2008
The light microscope is a basic tool for the cell biologist, who should have a thorough understanding of how it works, how it should be aligned for different applications, and how it should be maintained as required to obtain maximum image-forming capacity and resolution. The components of the microscope are described in detail here.
10:25
Single-Digit Nanometer Electron-Beam Lithography with an Aberration-Corrected Scanning Transmission Electron Microscope
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Cited by 4 •
2018
We use an aberration-corrected scanning transmission electron microscope to define single-digit nanometer patterns in two widely-used electron-beam resists: poly (methyl methacrylate) and hydrogen silsesquioxane. Resist patterns can be replicated in target materials of choice with single-digit nanometer fidelity using liftoff, plasma etching, and resist infiltration by organometallics.
FAQs
The lens system forms an enlarged image from light interacting with the specimen, while the camera sensor converts that image into digital data. Software then supports enlargement, measurement, storage, and analysis. This conversion makes the same inspection record available for later review, comparison, and communication among engineering teams.
The selected light path affects which features become visible: reflected light supports examination of surfaces and components, whereas transmitted light reveals information from samples that allow light to pass through. Controlled illumination and focus help produce interpretable images, allowing engineers to assess dimensions, material condition, or manufacturing defects more consistently.
Instead of limiting observation to a person looking through eyepieces, digital microscope imaging presents the magnified view on a screen. The digital format also enables image enlargement, measurement, storage, and analysis. These capabilities support documentation and collaboration, particularly when several people must examine the same component or inspection result.
An inspection can proceed by positioning the component or specimen, directing suitable reflected or transmitted illumination through the optical system, and adjusting focus until relevant features are clearly displayed. Engineers can then enlarge the image, examine surfaces or microstructures, and record measurements or observations for subsequent analysis and documentation.
Digital microscope imaging can support examination of surfaces, microstructures, electronic assemblies, and machined parts. It can also help reveal manufacturing defects and provide information about dimensions or material condition. These uses make the instrument relevant to component inspection, process-quality assessment, and evaluation of small-scale engineering features.
An inspection can produce an enlarged visual record that engineers store, measure, and analyze under controlled focus and illumination. The resulting information may help assess component dimensions, surface or material condition, microstructural features, and manufacturing defects. Stored images also improve documentation and make findings easier to share during engineering review or collaboration.