Dna Translocation
Dna Translocation - Related Videos
12:27
Measuring Peptide Translocation into Large Unilamellar Vesicles
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Cited by 9 •
2012
This protocol details a method for the quantitative measure of peptide translocation into large unilamellar lipid vesicles. This method also provides information about the rate of membrane translocation and can be used to identify peptides that efficiently and spontaneously cross lipid bilayers.
08:42
Determination of Zeta Potential via Nanoparticle Translocation Velocities through a Tunable Nanopore: Using DNA-modified Particles as an Example
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Cited by 7 •
2016
Here we use a polyurethane tunable nanopore integrated into a resistive pulse sensing technique to characterize nanoparticles surface chemistry via the measurement of particle translocation velocities, which can be used to determine the zeta potential of individual nanoparticles.
09:54
Live-imaging of PKC Translocation in Sf9 Cells and in Aplysia Sensory Neurons
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Cited by 14 •
2011
In this video, we demonstrate visualization of PKC translocation in living cells using fluorescently tagged PKCs.
10:27
Dual DNA Rulers to Study the Mechanism of Ribosome Translocation with Single-Nucleotide Resolution
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Cited by 1 •
2019
Dual DNA ruler assay is developed to determine the mRNA position during ribosome translocation, which relies on the dissociation forces of the formed DNA-mRNA duplexes. With single-nucleotide resolution and capability of reaching both ends of mRNA, it can provide mechanistic insights for ribosome translocation and probe other nucleic acid displacements.
05:39
Quantitative Measurement of GLUT4 Translocation to the Plasma Membrane by Flow Cytometry
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Cited by 20 •
2010
This protocol describes a rapid technique to quantify the translocation of GLUT4 from the cytoplasm to the plasma membrane of cells by flow cytometry.
FAQs
An applied electric field exerts force on the negatively charged DNA, driving it through a nanopore. As the molecule occupies the confined pathway, it temporarily changes the surrounding ionic current. Measuring that electrical disturbance converts an otherwise unseen transport event into a detectable signal for single-molecule sensing and analysis.
The observed speed depends on molecular length, pore geometry, and solution conditions. These variables influence how quickly DNA progresses through the confined pathway and therefore affect the timing of the associated ionic-current change. Engineering studies must account for them when comparing measurements or designing nanopore systems intended to detect transport consistently.
Ionic-current monitoring provides an indirect way to follow DNA as it moves through a nanoscale pathway. Each passage temporarily alters the current around the pore, producing information about molecular transport without requiring direct visual observation. This principle supports the development of single-molecule sensors and helps engineers evaluate how pore designs respond to biomolecular movement.
A basic workflow applies an electric field across a nanopore-containing system, allows DNA to move through the confined pathway, and records the resulting ionic-current changes. Engineers then relate those transient electrical signals to transport behavior, considering molecular length, pore geometry, and solution conditions. The workflow provides a foundation for studying and controlling nanoscale biomolecule movement.
Nanopore systems use the current changes produced during DNA passage as signals for single-molecule analysis. In sequencing-oriented designs, these transport-associated measurements support the examination of DNA as it moves through the pore. More broadly, the approach links molecular movement with electrical detection, enabling sensing technologies based on transient changes in ionic current.
Understanding DNA translocation supports the design of microfluidic devices, biosensors, and nanoscale platforms that control, detect, or sort biomolecules. The same transport principles help engineers connect pathway geometry and solution conditions with measurable molecular behavior. These applications are relevant when a device must handle very small biological targets with high sensitivity.