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Determination of Zeta Potential via Nanoparticle Translocation Velocities through a Tunable Nanopore: Using DNA-modified Particles as an Example
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Bioengineering
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JoVE Journal Bioengineering
Determination of Zeta Potential via Nanoparticle Translocation Velocities through a Tunable Nanopore: Using DNA-modified Particles as an Example

Determination of Zeta Potential via Nanoparticle Translocation Velocities through a Tunable Nanopore: Using DNA-modified Particles as an Example

DOI:
10.3791/54577-v

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08:42 min

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October 26, 2016

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, ,
1Department of Chemistry, School of Science,Loughborough University, 2Izon Science Limited, 3School of Mathematics and Physics,The University of Queensland

Chapters

  • 00:05Title
  • 01:07Preparing Streptavidin Coated Particles and Addition of Capture Probe DNA
  • 02:45Hybridizing Complementary DNA to the Capture Probe-particles
  • 03:46TRPS Setup
  • 04:58Calibrating the Nanopore for Zeta Analysis and Running a Sample
  • 06:43Results: Size and Zeta Potential of Streptavidin Coated Nanoparticles by TRPS
  • 07:47Conclusion

Summary

Automatic Translation

Automatic Translation

Here we use a polyurethane tunable nanopore integrated into a resistive pulse sensing technique to characterize nanoparticles surface chemistry via the measurement of particle translocation velocities, which can be used to determine the zeta potential of individual nanoparticles.

Tags

Keywords: Zeta PotentialNanoparticleTunable NanoporeDNA-modified ParticlesTRPSParticle-by-particle AnalysisBioassayStreptavidin-coated ParticlesDNA BindingMagnetic SeparationTarget DNA Binding

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