Sample Stage Leveling

Sample stage leveling is the process of aligning a specimen platform so its surface lies in a controlled, repeatable plane, which is essential for accurate measurement, imaging, and fabrication. The stage is leveled by adjusting its support points or actuators until a reference surface, alignment marker, or measurement signal indicates that tilt has been minimized relative to the instrument’s coordinate system. In engineering, this procedure improves focus consistency, dimensional accuracy, and repeatability in microscopy, inspection, machining, and experimental testing. Careful leveling also reduces geometric errors caused by sample tilt, supporting reliable comparisons across measurements and improving the performance of automated or precision instruments.

Sample Stage Leveling - Related Videos

Research

JoVE Journal - Biology

Blood Sampling and Hormone Measurement for Determining the Stage in the Ovarian Cycle in Marmosets

0 Views •

2024

This protocol describes blood and urine sampling for measuring progesterone/estradiol and chorionic gonadotropin levels to determine the ovarian cycle stage. The hormone levels are used to predict and determine the timing of ovulation and hormones are injected to regulate the ovarian cycle and oocyte growth.

Noninvasive Sampling of Mucosal Lining Fluid for the Quantification of In Vivo Upper Airway Immune-mediator Levels

0 Views •

Cited by 4 •

2017

This protocol describes a noninvasive technique for the sampling of undisturbed mucosal lining fluid from the upper airways. It can be used to perform the quantification of in vivo levels of protein mediators, such as cytokines and chemokines, in subjects of all ages.

Education

JoVE Core - Introduction to Psychology

High-Level and Low-Level Awareness

0 Views •

2024

Controlled processes in human consciousness represent high-alert mental states where individuals deliberately focus their attention on achieving specific goals. Controlled processes can be seen in situations like mastering new technology, where a person might become so absorbed that they ignore surrounding distractions. Such processes involve selective attention, requiring one to concentrate on particular elements of experience while disregarding others. These are governed by executive...

Education

JoVE Core - Analytical Chemistry
Free Sample

Leveling Effect

0 Views •

2024

In acid-base chemistry, the leveling effect refers to the limitation imposed by the solvent on the strength of acids and bases in solution. When a base stronger than the solvent's conjugate base is used, it deprotonates the solvent until the base is entirely consumed, making it ineffective against weaker acids. Conversely, an acid stronger than the solvent's conjugate acid protonates the solvent until the acid is depleted, rendering it ineffective against weaker bases. Essentially, the solvent...

Impact of High-intensity Interval Exercise and Moderate-Intensity Continuous Exercise on the Cardiac Troponin T Level at an Early Stage of Training

0 Views •

Cited by 3 •

2019

Here, we present protocols of high-intensity interval and moderate-intensity continuous exercise to observe the response of circulating cardiac troponin T (cTnT) concentration to acute exercise over 10 days. The information may assist with clinical interpretations of post-exercise cTnT elevation and guide the prescription of exercise.

View All Results

FAQs

Related Topics