Surface Profiling
Surface Profiling - Related Videos
15:17
Colorectal Cancer Cell Surface Protein Profiling Using an Antibody Microarray and Fluorescence Multiplexing
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Cited by 19 •
2011
We described a procedure for the disaggregation of colorectal cancer (CRC) to produce viable single cells, which are then captured on customized antibody microarrays recognizing surface antigens (DotScan CRC microarray). Sub-populations of cells bound to the microarray can be profiled by fluorescence multiplexing using monoclonal antibodies tagged with fluorescent dyes.
14:11
Quantification of Hydrogen Concentrations in Surface and Interface Layers and Bulk Materials through Depth Profiling with Nuclear Reaction Analysis
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Cited by 21 •
2016
We illustrate the application of 1H(15N,αγ)12C resonant nuclear reaction analysis (NRA) to quantitatively evaluate the density of hydrogen atoms on the surface, in the volume, and at an interfacial layer of solid materials. The near-surface hydrogen depth profiling of a Pd(110) single crystal and of SiO2/Si(100) stacks is described.
08:07
Profiling of Surface Protein Epitopes on Viral Particles by Multiplex Dual-Reporter Strategy
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2024
Here, we describe a newly developed multiplex fluorescent immunoassay that uses a dual-reporter flow cytometric system to concurrently detect two unique spike protein epitopes on intact severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) viral particles that had been captured by angiotensin-converting enzyme-2-coupled magnetic microspheres.
07:13
Using Topographic Maps to Generate Topographic Profiles
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2023
Source: Laboratory of Alan Lester - University of Colorado Boulder Topographic maps are "plan-view" representations of Earth's three-dimensional surface. They are a standard type of map-view that provides an overhead, or aerial, perspective. Among the defining features of a topographic map are the contour lines that indicate locations of constant elevation. The elevation interval between the contour lines is dependent on the level of detail provided by the map and the kind of topography present.
08:29
Expression Profiling with Microarrays
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2023
Microarrays are important tools for profiling gene expression, and are based on complementary binding between probes that are attached to glass chips and nucleic acids derived from samples. Using these arrays, scientists can simultaneously evaluate the expression of thousands of genes. In addition, the expression profiles of different cells or tissue types can be compared, allowing researchers to deduce how the expression of different genes change during biological processes, and thus gain...
FAQs
Surface profiling converts measured height changes into a topographic map and numerical descriptors, allowing engineers to examine more than visual appearance. Roughness, waviness, texture, and defects can be considered as surface characteristics, while parameters such as Ra and Rz provide quantitative values for comparing parts, processes, or conditions.
A stylus system records geometry as a physical tip traces the surface, whereas an optical system derives height information from reflected light or interference patterns. Both approaches produce a scan that can be converted into a topographic map, but the measurement mechanism differs. Engineers can select the acquisition approach that fits the surface and inspection objective.
Numerical parameters such as Ra and Rz make surface comparisons more consistent than relying only on visual inspection. Their values can be related to functional concerns including wear, friction, sealing, and contact behavior. This connection helps engineers judge whether a measured surface is likely to support intended service performance and identify changes requiring process attention.
A basic workflow begins by choosing stylus or optical acquisition, scanning the relevant surface, converting the measurement into a topographic map, and calculating quantitative descriptors. The resulting data can then be compared with manufacturing conditions or used to assess performance-related characteristics. This sequence links measurement to quality control and process optimization rather than treating the scan as an isolated image.
Machining optimization and coating assessment benefit from surface profiling because the measurements connect manufactured surfaces with measurable geometry. Engineers can use the results in quality control, evaluate consistency across parts, and examine whether processing changes alter surface characteristics relevant to service. This makes the technique useful for improving process consistency as well as finished-part performance.
Engineers should use surface profiling when surface condition may influence reliability, performance, or consistency in service. The measurements can support investigations of wear, friction, sealing, and contact behavior, while also showing how manufacturing conditions affect the finished surface. These outcomes help connect inspection data with practical decisions about process control and component suitability.