Suture Degradation
Suture Degradation - Related Videos
02:58
Regulated Protein Degradation
0 Views •
2020
It is vital to regulate the activity of enzymatic as well as non-enzymatic proteins inside the cell. This can be achieved either through creating a balance between their rate of synthesis and degradation or regulating the intrinsic activity of the protein. Both these regulation mechanisms play an essential role in the normal functioning of cells. Protein degradation plays two important roles in the cells. It helps to protect cells from misfolded or damaged proteins before they lead to a...
02:11
Proteins: From Genes to Degradation
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2020
Within a biological system, the DNA encodes the RNA, and the nucleotide sequence in the RNA further defines the amino acid sequence in the protein. This is referred to as “The Central Dogma of Molecular Biology” - a term coined by Francis Crick. Central dogma is a firm principle in biology that defines the flow of genetic information within any life form. The two fundamental steps in central dogma are - transcription and translation. Transcription is the synthesis of RNA molecules by RNA...
04:51
Q Suture Technique: A Suturing Technique to Reduce Gap Formation and Increase Tensile Strength in Tendon Repair
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2025
In this video, we demonstrate suturing techniques — core suture and Q suture — to repair the foot flexor tendon in an ex vivo porcine model. Q sutures enhance the strength of the core suture in tendon repair and decrease gapping at the tendon repair site.
07:43
Assaying Proteasomal Degradation in a Cell-free System in Plants
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Cited by 26 •
2014
Targeted protein degradation represents a major regulatory mechanism for cell function. It occurs via a conserved ubiquitin-proteasome pathway, which attaches polyubiquitin chains to the target protein that then serve as molecular “tags” for the 26S proteasome. Here, we describe a simple and reliable cell-free assay for proteasomal degradation of proteins.
10:56
Assays for the Degradation of Misfolded Proteins in Cells
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Cited by 5 •
2016
This report describes protocols for measuring degradation rates of misfolded proteins by either western blot or fluorescence-based assays. The methods can be applied to analysis of other misfolded proteins and for high throughput screening.
FAQs
These mechanisms reduce the integrity of the suture through different degradation pathways. Hydrolysis uses water to cleave polymer bonds, while enzymatic and cellular activity can progressively change the material through biological interactions. As degradation proceeds, molecular weight, mass, and tensile strength decrease, which can reduce the material’s ability to support a repair.
Molecular weight provides an indication of how extensively polymer chains have been disrupted. A decline in molecular weight signals that degradation has altered the material structure, even before all visible mass has disappeared. Tracking this change alongside tensile strength and mass loss helps engineers relate molecular-level damage to the functional persistence of a suture.
A strength-retention profile shows how mechanical support changes as degradation progresses. It helps identify whether a material maintains useful tensile strength during the period when a repair requires support. Engineers can compare this profile with tissue-healing needs, making it a practical measure for judging whether a suture’s persistence is appropriately matched to its intended function.
Mass loss and structural changes provide information that tensile testing alone may not capture. A suture can undergo changes in polymer structure and molecular weight while its remaining mass and mechanical performance evolve differently. Measuring these properties together gives a broader engineering picture of degradation and supports more informed evaluation under relevant physiological conditions.
Engineering evaluations commonly examine strength-retention profiles, mass loss, and changes in material structure. These measurements can be considered over the course of degradation under relevant physiological conditions. Together, they indicate how the suture’s mechanical behavior, physical persistence, and internal material characteristics change, providing evidence for selecting or designing an appropriate surgical material.
Degradation data help engineers design absorbable sutures whose material persistence corresponds to the expected rate of tissue healing. A suitable design must retain enough mechanical support while the repair needs reinforcement, then progressively lose material as healing advances. Strength retention, mass loss, and structural change therefore become design criteria rather than isolated laboratory observations.
The same degradation principles apply to tissue engineering and biomedical devices. In each setting, engineers need to understand how material structure, mass, and tensile strength change under relevant physiological conditions. These evaluations help determine whether a degradable material will maintain its intended role for an appropriate period before its mechanical support and physical persistence decline.