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JoVE Journal
Neuroscience
Author Produced

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Cryopreservation of Cortical Tissue Blocks for the Generation of Highly Enriched Neuronal Cultures
 

Cryopreservation of Cortical Tissue Blocks for the Generation of Highly Enriched Neuronal Cultures

Article DOI: 10.3791/2384
November 11th, 2010

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Summary November 11th, 2010

Here, we describe a method for efficient cryopreservation and thawing of cortical brain tissue blocks to generate highly enriched neuronal cultures. This simple protocol provides flexibility for later generation of neuronal, astrocyte, and neuronal precursor cell cultures.

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