Hep G2 Cells
Hep G2 Cells - Related Videos
13:01
Anti-Nuclear Antibody Screening Using HEp-2 Cells
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Cited by 34 •
2014
Indirect immunofluorescent (IIF) assays have traditionally been used for the detection of antinuclear antibodies (ANA) in human serum. The presence of these antibodies can aid in the diagnosis of systemic autoimmune rheumatic diseases (SARD). This protocol demonstrates how to effectively perform the IIF technique to accurately detect these autoantibodies.
06:40
G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome
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2018
We describe a technique for combining flow cytometry and high throughput sequencing to identify late replicating regions of the genome.
09:32
Drug-Induced Senescence in Liver Cells Promotes M2 Macrophage Polarization: Implications for Tyrosine Kinase Inhibitor-Associated Hepatotoxicity
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2025
Here, we study hepatotoxicity caused by lapatinib and neratinib through SASP-driven macrophage polarization, which offers a deeper mechanistic understanding beyond traditional toxicity tests. This approach demonstrates how senescent hepatocytes influence immune responses. This is particularly valuable for assessing long-term drug effects and discovering new therapeutic targets for managing drug-induced hepatotoxicity.
09:13
Study of Viral Vectors in a Three-dimensional Liver Model Repopulated with the Human Hepatocellular Carcinoma Cell Line HepG2
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Cited by 7 •
2016
The recellularized extracellular matrix of a decellularized rat liver can be used as a humanized, three-dimensional ex vivo model to study the distribution and transgene expression of a virus or viral vector.
10:05
Simultaneous Distinction of Monospecific and Mixed DFS70 Patterns During ANA Screening with a Novel HEp-2 ELITE/DFS70 Knockout Substrate
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Cited by 5 •
2018
DFS70 autoantibodies mimic common disease-associated antinuclear antibody patterns making accurate interpretation challenging when using conventional HEp-2 substrates. The protocol describes advantages of novel engineered HEp-2 substrate over conventional HEp-2 in ANA screening and distinguishing DFS70 patterns with high confidence in both monospecific and mixed ANA positive cases.
FAQs
Researchers can examine coordinated changes in cell viability, oxidative stress, membrane integrity, and gene expression after chemical exposure. Considering these endpoints together helps distinguish broad cellular damage from more specific biological responses. This approach can indicate how a pollutant affects liver-derived cells and identify mechanisms that may contribute to chemical toxicity relevant to human health.
Oxidative stress measurements show whether an environmental chemical is associated with cellular stress, while gene-expression measurements reveal changes in the cell’s molecular response. These endpoints add mechanistic detail beyond viability alone. Comparing them with membrane-integrity results can help researchers characterize the pattern of injury produced by pesticides, industrial contaminants, pharmaceuticals, or nanomaterials.
Hep G2 experiments provide an in vitro view of responses in human liver-derived cells, whereas animal and ecological assessments address broader biological or environmental effects. The cell model can therefore support early toxicity screening and mechanistic analysis without replacing those other approaches. Results may help prioritize substances for further testing across complementary assessment systems.
A typical study maintains Hep G2 cells as adherent cultures, exposes them to the environmental chemical of interest, and then measures selected cellular responses. Common readouts include viability, oxidative stress, membrane integrity, and gene expression. The resulting pattern is evaluated to determine whether the substance produces cellular effects and which responses warrant additional investigation.
The model supports screening of several environmentally relevant substance categories, including pesticides, industrial contaminants, pharmaceuticals, and nanomaterials. Researchers can compare the cellular responses associated with these different exposures using the same general set of toxicity-related endpoints. This broad applicability makes the system useful for identifying substances that merit more detailed toxicological assessment.
Findings can identify cellular mechanisms associated with chemical injury, indicate whether exposure affects viability or other measured responses, and help prioritize substances for further testing. In environmental research, this information complements animal and ecological assessments by adding human liver-cell evidence. The combined evidence can strengthen evaluation of potential risks posed by environmental chemicals.