Flow Injection

Flow injection is an automated analytical technique in which a sample is introduced into a continuously moving carrier stream and measured as it passes through a detection system. After injection, controlled dispersion mixes the sample with reagents in tubing, allowing chemical reactions to develop before a detector records a transient signal related to analyte concentration. In environmental analysis, flow injection supports rapid, reproducible measurements of nutrients, metals, and other contaminants in water and related samples. Its continuous operation can reduce manual handling, reagent consumption, and analysis time, making it useful for routine monitoring and high-throughput assessment of environmental quality.

Flow Injection - Related Videos

Research

JoVE Journal - Developmental Biology

In Utero Intra-cardiac Tomato-lectin Injections on Mouse Embryos to Gauge Renal Blood Flow

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Cited by 2 •

2015

This manuscript describes a technique for visualization of the developing vasculature. Here we utilized in utero intra-cardiac FITC-labeled tomato lectin microinjections on mouse embryos. Using this technique, we delineate the perfused and unperfused vessels throughout the embryonic kidney.

Flow Cytometry Purification of Mouse Meiotic Cells

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Cited by 65 •

2011

An efficient method to obtain highly purified viable meiotic fractions from mouse testis is described, which combines a refined cell dissociation protocol with fluorescent activated cell sorting (FACS). This method takes advantage of differences in the DNA content and nuclear density of discrete meiotic fractions.

Automation of the Micronucleus Assay Using Imaging Flow Cytometry and Artificial Intelligence

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Cited by 5 •

2023

The micronucleus (MN) assay is a well-established test for quantifying DNA damage. However, scoring the assay using conventional techniques such as manual microscopy or feature-based image analysis is laborious and challenging. This paper describes the methodology to develop an artificial intelligence model to score the MN assay using imaging flow cytometry data.

Zebrafish Brain Ventricle Injection

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Cited by 72 •

2009

After neural tube formation, the neuroepithelium constricts and folds while the tube fills with embryonic cerebrospinal fluid (eCSF) to form the embryonic brain ventricles. We developed this ventricle injection technique to better visualize the fluid filled space in contrast to the neuroepithelial shape in a live embryo.

Intraductal Injection: Delivering Injection Mix into the Ducts of the Mouse Mammary Gland

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2023

This video describes an intraductal injection technique where we deliver injection mix into the ducts of mouse mammary glands.

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