Laboratory Screening Method
Laboratory Screening Method - Related Videos
18:00
Aseptic Laboratory Techniques: Plating Methods
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Cited by 438 •
2012
When working with media and reagents used to culture microorganisms, aseptic technique must be practiced to ensure contamination is minimized. A variety of plating methods are routinely used to isolate, propagate, or enumerate bacteria and phage, all of which incorporate procedures that maintain the sterility of experimental materials.
06:40
A Robust Method for the Large-Scale Production of Spheroids for High-Content Screening and Analysis Applications
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Cited by 4 •
2021
This protocol details a method for the production of three different types of spheroids in a manner that makes them suitable for large-scale high-content screening and analysis. In addition, examples are presented showing how they can be analyzed at spheroid and individual cell levels.
10:49
Laboratory-Engineered Glioblastoma Organoid Culture and Drug Screening
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2025
Here, we outline a comprehensive protocol for generating and utilizing laboratory-engineered glioblastoma organoids (LEGO) to investigate genotype-phenotype dependencies and screen potential drugs for glioblastoma treatment.
14:13
Detecting Estrogenic Ligands in Personal Care Products using a Yeast Estrogen Screen Optimized for the Undergraduate Teaching Laboratory
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Cited by 1 •
2018
This article presents an optimized yeast estrogen screen for quantifying ligands in Personal Care Products (PCPs) that bind estrogen receptors alpha (ERα) and/or beta (ERβ). The method incorporates two colorimetric substrate options, a six-day refrigerated incubation for use in undergraduate courses, and statistical tools for data analysis.
06:49
A Laboratory Method to Measure Contagious Yawning in Rats
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Cited by 1 •
2019
The method described here aims to obtain yawn contagion curves in pairs of familiar or unfamiliar male rats. Cages with holes separated by either clear or opaque partitions with (or without) holes are used to detect whether visual, olfactory, or both types of sensory cues can stimulate yawn contagion.
FAQs
The decision comes from comparing an observed sample signal with a reference basis rather than interpreting the signal in isolation. Reference materials, calibration data, or defined screening thresholds provide context for judging the result. This comparison supports consistent decisions across samples and helps distinguish potential findings that warrant follow-up from results that do not require immediate additional analysis.
The assay determines how the sample is examined and what kind of signal becomes available for comparison. Depending on the target substance, organism, or property, a chemical, biological, or instrumental approach may be used. Selecting among these approaches allows the screening process to address different environmental questions involving pollutants or changes in soil, water, and sediment.
Screening provides an efficient first evaluation that helps identify samples needing closer examination, whereas confirmatory testing represents the more detailed follow-up stage. The initial result does not replace that subsequent analysis. Instead, it helps researchers prioritize limited time and resources toward samples showing signals associated with potential contamination or other relevant environmental changes.
A practical workflow begins with collecting the relevant soil, water, or sediment sample and preparing it for examination. An appropriate chemical, biological, or instrumental assay is then applied, and the resulting signal is compared with reference materials, calibration data, or a defined threshold. Samples identified as important can be prioritized for confirmatory testing.
The approach can be applied to soil, water, and sediment when researchers need to examine potential pollutants or detect changes in environmental conditions. Its value lies in handling these questions consistently across many samples. The resulting comparisons help indicate which samples deserve detailed investigation, supporting broader contamination assessment and environmental monitoring efforts.
It is especially useful when many samples must be assessed efficiently before detailed analysis is attempted on every sample. Screening can support contamination assessment, regulatory evaluation, and targeted follow-up investigations by identifying priority samples. In this context, it improves the organization of environmental studies and helps direct confirmatory work toward the most relevant findings.