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Chapters
- 00:05Introduction
- 00:35Coating 96-Well Plate with ECM Basement Material
- 01:07Subculturing and Cell Counting
- 01:52Seeding Cells into ECM Basement Material-Coated Plates
- 02:41Image Acquisition of Spheroids
- 03:07Image Acquisition of Spheroids
- 04:35Results: Observation and Imaging of Spheroids
- 06:07Conclusion
This protocol details a method for the production of three different types of spheroids in a manner that makes them suitable for large-scale high-content screening and analysis. In addition, examples are presented showing how they can be analyzed at spheroid and individual cell levels.
