Plant Disease Detection

Plant disease detection is the process of identifying infections or disorders that impair plant health, an essential step for protecting crops, ecosystems, and environmental resources. It works by examining symptoms such as discoloration, lesions, wilting, or abnormal growth, often supplemented by microscopy, molecular assays, spectral imaging, or machine-learning analysis of plant images. Early, accurate detection helps distinguish biotic diseases from abiotic stress, guide targeted treatment, and limit unnecessary pesticide use. In environmental research, these methods support monitoring of vegetation health, assessment of disease spread, and development of sustainable strategies for managing agricultural and natural plant communities.

Plant Disease Detection - Related Videos

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JoVE Journal - Biology
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Detection of Histone Modifications in Plant Leaves

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Cited by 22 •

2011

A reliable and useful approach to detect histone modifications on specific plant genes is described. The approach combines chromatin immunoprecipitation (ChIP) and real-time quantitative PCR. It allows detection of histone modifications on specific genes with a role in diverse physiological processes.

Microwave Assisted Rapid Diagnosis of Plant Virus Diseases by Transmission Electron Microscopy

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Cited by 16 •

2011

This study describes a method that allows the rapid and clear diagnosis of plant virus diseases in about half a day by using a combination of microwave assisted plant sample preparation for transmission electron microscopy and negative staining methods.

Research

JoVE Journal - Biology

Detecting Virus and Salivary Proteins of a Leafhopper Vector in the Plant Host

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Cited by 1 •

2021

This protocol demonstrates how to use the plant host to detect salivary proteins of leafhopper and plant viral proteins released by leafhopper vectors.

Detection of Protein Interactions in Plant using a Gateway Compatible Bimolecular Fluorescence Complementation (BiFC) System

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Cited by 30 •

2011

We have developed a technique to test protein-protein interactions in plant. A yellow fluorescent protein (YFP) is split into two non-overlapping fragments. Each fragment is cloned in-frame to a gene of interest via Gateway system, enabling expression of fusion proteins. Reconstitution of YFP signal only occurs when the inquest proteins interact.

Research

JoVE Journal - Biology
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Transmitting Plant Viruses Using Whiteflies

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Cited by 41 •

2013

Improving our understanding of and our ability to manage many of the insect-transmitted plant viruses requires the use of the vector. Insect transmission of plant viruses is a tritrophic interaction, and as such requires the manipulation of insects, virus, and plant. Vectors must be reared in large numbers and manipulated in such a way as to insure high rates of transmission to test plants. The basics of rearing and manipulating the whitefly, Bemisa tabaci (Hemiptera: Aleyrodidae), a vector of...

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