Adult Mice
Adult Mice - Related Videos
09:35
Environmental Modulations of the Number of Midbrain Dopamine Neurons in Adult Mice
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Cited by 12 •
2015
This protocol describes two different environmental manipulations and a concurrent brain infusion protocol to study environmentally-induced brain changes underlying adaptive behavior and brain repair in adult mice.
08:34
Isolation of Atrial Myocytes from Adult Mice
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Cited by 9 •
2019
This protocol is used to isolate single atrial cardiomyocytes from the adult mouse heart using a chunk digestion approach. This approach is used to isolate right or left atrial myocytes that can be used to characterize atrial myocyte electrophysiology in patch-clamp studies.
09:52
One Mouse, Two Cultures: Isolation and Culture of Adult Neural Stem Cells from the Two Neurogenic Zones of Individual Mice
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Cited by 154 •
2014
Here we describe a detailed protocol for the simultaneous generation of neural precursor cell cultures, as either adherent monolayers or neurospheres, from the subventricular zone and dentate gyrus of individual adult mice.
09:32
Methods for the Isolation, Culture, and Functional Characterization of Sinoatrial Node Myocytes from Adult Mice
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Cited by 25 •
2016
Methods are demonstrated for the isolation of sinoatrial node myocytes (SAMs) from adult mice for patch clamp electrophysiology or imaging studies. Isolated cells can be used directly or can be maintained in culture to permit expression of proteins of interest, such as genetically encoded reporters.
08:32
Subpial Adeno-associated Virus 9 (AAV9) Vector Delivery in Adult Mice
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Cited by 9 •
2017
The goal of the present study was to develop and validate the potency and safety of spinal adeno-associated virus 9 (AAV9)-mediated gene delivery by using a novel subpial gene delivery technique in adult mice.
FAQs
Investigators connect inherited alleles with adult phenotypes by comparing genetic differences with measurable traits or molecular responses. An allele is a genetic variant, and its effect can be assessed when genotype and phenotype are examined together. This approach helps distinguish gene-associated effects from unrelated variation and clarifies how particular genetic differences contribute to biological characteristics.
Controlled breeding creates defined patterns of inheritance that allow investigators to follow alleles across generations. Researchers can then compare adult offspring with different inherited genetic combinations and determine whether particular traits or molecular responses track with those combinations. This provides a structured basis for evaluating genetic contributions rather than relying only on naturally occurring variation.
Environmental conditions can influence the traits and molecular responses observed alongside inherited alleles. For that reason, investigators interpret adult phenotypes in relation to both genetic background and environmental exposure. Considering these factors is important when assessing disease susceptibility, physiology, behavior, or other outcomes that may reflect interactions between genetic contributions and surrounding conditions.
A typical workflow combines controlled breeding, genotyping, and analysis of adult traits or molecular responses. Genotyping identifies inherited alleles, while organism-level observations, tissue examination, or molecular measurements reveal associated outcomes. Comparing these data enables investigators to connect genetic variation with gene function and determine whether a candidate allele corresponds to a specific phenotype.
Adult tissues can provide evidence at several biological levels. Investigators may examine tissue-level characteristics and molecular responses, then relate those findings to the animal’s genotype and broader phenotype. This layered analysis helps show whether a genetic difference affects physiology or disease-related biology through changes that are detectable beyond the whole-animal level.
They are especially useful when researchers need to evaluate how genetic differences influence physiology, behavior, disease susceptibility, or responses to environmental conditions after juvenile development. Combining genotype data with adult organismal, tissue, and molecular observations supports gene-function validation and helps assess genetic contributions to complex biological processes and disease.