Crispr Insertion
Crispr Insertion - Related Videos
01:59
CRISPR
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2019
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced Short...
02:53
CRISPR and crRNAs
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2020
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea. The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
11:53
Substrate Generation for Endonucleases of CRISPR/Cas Systems
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Cited by 4 •
2012
CRISPR/Cas systems mediate adaptive immunity in Bacteria and Archaea. Many Cas proteins are proposed to act as endoribonucleases acting on crRNA precursors of varying length. Here we illustrate three different approaches to generate pre-crRNA substrates for the biochemical analysis of Cas endonuclease activity.
02:55
Genetically Modifying CAR T Cells Using a CRISPR-Cas9 System
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2025
The video outlines a process for creating genetically modified CAR T cells through the CRISPR-Cas9 System. Infecting T cells with CRISPR and CAR lentiviruses results in modifications to the target gene and the synthesis of a chimeric antigen receptor or CAR, ultimately leading to the formation of genetically modified CAR T cells.
04:22
A Technique for Gene Editing in Natural Killer Cells Using CRISPR Cas9
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2025
This video demonstrates a technique for Cas9 ribonucleoprotein-mediated genetic modification of primary natural killer (NK) cells. A Cas9 ribonucleoprotein, consisting of a Cas9 endonuclease bound to a guide RNA (gRNA) formed by base pairing a CRISPR RNA (crRNA) and a trans-activating crRNA (tracrRNA), is introduced into primary natural killer cells via electroporation. The ribonucleoprotein targets and cleaves the host DNA at the target site, leading to gene knockout via modification of the...
FAQs
The guide RNA provides the targeting information by pairing with a complementary genomic sequence. This pairing positions the CRISPR-associated nuclease, such as Cas9, at the selected locus, where it creates a double-strand break. Because the guide RNA is designed around the intended genomic site, its sequence is central to directing insertion to a specific location rather than elsewhere in the genome.
The donor DNA template supplies the sequence intended for incorporation at the nuclease-generated break. Homology-directed repair uses this template to guide replacement or addition of genetic material at the targeted locus. Its role enables planned changes such as adding a tag, correcting a disease-associated variant, or introducing another chosen sequence rather than relying only on the break itself.
Homology-directed repair provides the repair pathway through which a supplied donor sequence can be incorporated after Cas9 creates a double-strand break. The process connects the engineered DNA with the selected genomic site, allowing a designed sequence change to become part of the genome. This mechanism is especially relevant when the goal requires a defined insertion rather than an unspecified repair outcome.
A typical workflow begins by selecting a genomic site and designing a guide RNA with complementarity to that region. A CRISPR-associated nuclease is then directed to the site to create a double-strand break, while a donor DNA template supplies the desired sequence. Homology-directed repair can subsequently incorporate that template, producing the planned genetic modification.
CRISPR insertion can support gene tagging, correction of disease-associated variants, functional analysis of regulatory regions, and introduction of new traits. These applications allow investigators to connect specific DNA changes with gene activity or observable characteristics. In genetics research, the method therefore serves both as a tool for testing gene function and as a way to create defined alterations in cells or model organisms.
Researchers may choose this approach when they need to examine the effect of a precisely placed DNA change in cells or model organisms. It can help investigate gene function, regulatory regions, and disease-associated variants, while also supporting development of potential therapeutic strategies. The resulting targeted modifications provide a way to relate a selected genetic alteration to biological outcomes.