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JoVE Journal
Biology

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Radioactive in situ Hybridization for Detecting Diverse Gene Expression Patterns in Tissue
 

Radioactive in situ Hybridization for Detecting Diverse Gene Expression Patterns in Tissue

Article DOI: 10.3791/3764
April 27th, 2012

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Summary April 27th, 2012

This protocol is successfully used to quantitatively detect levels and spatial patterns of mRNA expression in multiple tissue types across vertebrate species. The method can detect low abundance transcripts and allows processing of hundreds of slides simultaneously. We present this protocol using expression profiling of avian embryonic brain formation as an example.

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