Amoeba Viability
Amoeba Viability - Related Videos
04:15
Detecting Bacterial Virulence Using Amoeba as a Host System
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2026
Source: Dubois, V., et al. Identification of Virulence Markers of Mycobacterium abscessus for Intracellular Replication in Phagocytes. J. Vis. Exp. (2018)This video demonstrates the procedure of co-culturing amoebae with mutant bacterial strains to evaluate their intracellular survival and identify virulence markers through differences in colony counts.
03:25
Amoeba-Based Enrichment of Giant Viruses from an Environmental Water Sample
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2026
Source: Bou Khalil, J. Y.,et al. A Rapid Strategy for the Isolation of New Faustoviruses from Environmental Samples Using Vermamoeba vermiformis. J. Vis. Exp. (2016)This video demonstrates the enrichment of giant viruses from an environmental water sample using amoeba-based co-culture to enable downstream detection and analysis.
02:04
Transfection of Wild-Type Amoeba via Electroporation with Bacterial Feeding Support
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2025
Source: Paschke, P., et al. Genetic Engineering of Dictyostelium discoideum Cells Based on Selection and Growth on Bacteria. J. Vis. Exp. (2019).This video demonstrates the procedure for introducing a plasmid carrying a target gene into amoeba cells via electroporation, followed by recovery in a bacterial supplement to enable efficient gene delivery in wild-type amoeba.
04:12
Isolation of a Single Giant Virus from a Mixed Viral Population Using Amoebae
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2026
Source: Sahmi-Bounsiar, D. et al. Single Cell Micro-aspiration as an Alternative Strategy to Fluorescence-activated Cell Sorting for Giant Virus Mixture Separation. J. Vis. Exp. (2019)This video demonstrates single-cell micro-aspiration to isolate and expand a single giant virus population from a mixed viral population using amoebae as host cells.
12:03
Viability Assays for Cells in Culture
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Cited by 39 •
2014
Therapeutic compounds are often first examined in vitro with viability assays. Blind cell counts by a human observer can be highly sensitive to small changes in cell number but do not assess function. Computerized viability assays, as described here, can assess both structure and function in an objective manner.
FAQs
No single indicator fully captures amoeba status. Membrane integrity indicates whether the cell boundary remains intact, whereas metabolic activity shows ongoing cellular function. Morphology can reveal visible damage or abnormality, and recovery or proliferation after stress tests whether the population can resume growth. Using these readouts together helps distinguish superficially intact cells from cells that remain functionally active.
A post-stress recovery assessment asks whether amoebae can regain cellular function after an environmental change or antimicrobial exposure. This adds information beyond a measurement taken immediately after treatment, because cells that appear damaged may differ in their capacity to recover. Tracking recovery or proliferation therefore helps evaluate the lasting effect of stress and identify surviving populations.
Amoebae may be examined under defined conditions that represent environmental changes or host-associated settings. Those conditions can influence whether cells maintain membrane integrity, metabolic activity, morphology, or the ability to recover. Interpreting viability in relation to the surrounding conditions helps researchers characterize how amoebae persist and how environmental shifts may affect infection-related observations.
A basic assessment establishes defined conditions, exposes amoebae to the environmental change or antimicrobial treatment of interest, and measures one or more viability indicators. Researchers may examine membrane integrity, metabolic activity, morphology, or recovery and proliferation after stress. Comparing these outcomes provides a structured view of cellular damage, continued function, and the potential for persistence.
Viability measurements show whether an antimicrobial treatment leaves amoebae metabolically active, morphologically intact, damaged, or unable to recover and proliferate. Examining several indicators can distinguish cells that merely retain some structural features from those that remain functional. This helps researchers characterize treatment effectiveness and assess whether surviving amoebae could remain relevant to infection studies.
In immunology and infection research, viability data help separate surviving pathogens from damaged or inactive cells. That distinction supports analysis of host-pathogen interactions, including whether amoebae can persist in host-associated environments. The findings also inform evaluations of infection risk and research strategies designed to control amoeba-associated disease by linking cellular status with potential biological relevance.